干旱区生态环境知识资源中心(Arid): Comparative metagenomics of two distinct biological soil crusts in the Tengger Desert, China

Arid

DOI	10.1016/j.soilbio.2019.107637
	Comparative metagenomics of two distinct biological soil crusts in the Tengger Desert, China
	Li, Jing-Yu 1,2; Jin, Xin-Ying 1; Zhang, Xiao-Chong 1; Chen, Long 3; Liu, Jian-Li 1; Zhang, Hui-Min 3; Zhang, Xiu 1,2; Zhang, Yi-Fei 4; Zhao, Jian-Hua 3; Ma, Zhi-Shan 1; Jin, Duo 1
通讯作者	Li, Jing-Yu
来源期刊	SOIL BIOLOGY & BIOCHEMISTRY
ISSN	0038-0717
出版年	2020
卷号	140
英文摘要	Biological soil crusts (biocrusts) are widely distributed in arid and semi-arid environments worldwide. The microbiome metabolic pathways of biocrusts contribute to important biogeochemical processes that improve the physicochemical properties of the surface soil. However, the differences in microbial processes among different types of biocrusts have been poorly studied by metagenomic analyses. In this study, we compared two types of biocrusts (bacterial-dominated biocrusts and moss-dominated biocrusts) using shotgun metagenomic sequencing. Our results showed that Actinobacteria was the most abundant phylum in the microbiomes of bacterial and moss biocrusts, even though the two biocrusts differed in the composition of the following other abundant phyla Proteobacteria, Acidobacteria, Cyanobacteria, Planctomycetes and Bacteroidetes. The profile of the C- and N- related metabolic pathways of the metagenome differed between the bacterial and moss biocrusts. The results showed that the genes encoding carbon monoxide dehydrogenase were more abundant than the gene encoding the ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) involved in the Calvin cycle, which is actually the main metabolic pathway for carbon fixation in both biocrusts. Low diversity in N-2 fixation pathways appears to be a distinguishing feature of biocrust microbiomes, while nitrogen reduction dominated the metabolic networks of the two biocrusts. The microbiome of bacterial biocrusts rich in assimilatory nitrate reduction genes, while the microbiome of moss biocrusts was rich in dissimilatory nitrate reduction genes. Amongst the (bio-)chemical parameters it is total organic carbon(TOC) that differentiates between the bacterial and moss biocrusts. Our study suggests that the biocrust types have significant effects on the TOC content and taxonomic, gene and metabolic diversity.
英文关键词	Biocrusts Microbiome Metabolism pathways Biogeochemical cycle
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000501414200005
WOS关键词	SUCCESSIONAL STAGES ; BACTERIAL COMMUNITIES ; NITROGEN-FIXATION ; EXTRACTION METHOD ; COLORADO PLATEAU ; CARBON FIXATION ; LOESS PLATEAU ; SINGLE-CELL ; SAND DUNES ; DIVERSITY
WOS类目	Soil Science
WOS研究方向	Agriculture
EI主题词	2020-01-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/312279
作者单位	1.North Minzu Univ, Coll Biol Sci & Engn, Xixia Dist 750021, Yinchuan, Peoples R China; 2.North Minzu Univ, Ningxia Key Lab Microbial Resource Dev & Applicat, Yinchuan 750021, Peoples R China; 3.Shanghai Majorbio Biopharm Biotechnol Co Ltd, Shanghai 201203, Peoples R China; 4.Inner Mongolia Acad Environm Sci, Hohhot 010010, Peoples R China
推荐引用方式 GB/T 7714	Li, Jing-Yu,Jin, Xin-Ying,Zhang, Xiao-Chong,et al. Comparative metagenomics of two distinct biological soil crusts in the Tengger Desert, China[J],2020,140.
APA	Li, Jing-Yu.,Jin, Xin-Ying.,Zhang, Xiao-Chong.,Chen, Long.,Liu, Jian-Li.,...&Jin, Duo.(2020).Comparative metagenomics of two distinct biological soil crusts in the Tengger Desert, China.SOIL BIOLOGY & BIOCHEMISTRY,140.
MLA	Li, Jing-Yu,et al."Comparative metagenomics of two distinct biological soil crusts in the Tengger Desert, China".SOIL BIOLOGY & BIOCHEMISTRY 140(2020).