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DOI	10.1146/annurev-ento-011019-025224
	Mechanisms, Applications, and Challenges of Insect RNA Interference
	Zhu, Kun Yan 1; Palli, Subba Reddy 2
通讯作者	Zhu, Kun Yan
来源期刊	ANNUAL REVIEW OF ENTOMOLOGY, VOL 65
ISSN	0066-4170
EISSN	1545-4487
出版年	2020
卷号	65 页码:293-311
英文摘要	The RNA interference (RNAi) triggered by short/small interfering RNA (siRNA) was discovered in nematodes and found to function in most living organisms. RNAi has been widely used as a research tool to study gene functions and has shown great potential for the development of novel pest management strategies. RNAi is highly efficient and systemic in coleopterans but highly variable or inefficient in many other insects. Differences in double-stranded RNA (dsRNA) degradation, cellular uptake, inter- and intracellular transports, processing of dsRNA to siRNA, and RNA-induced silencing complex formation influence RNAi efficiency. The basic dsRNA delivery methods include microinjection, feeding, and soaking. To improve dsRNA delivery, various new technologies, including cationic liposome assisted, nanoparticle-enabled, symbiont-mediated, and plant-mediated deliveries, have been developed. Major challenges to widespread use of RNAi in insect pest management include variable RNAi efficiency among insects, lack of reliable dsRNA delivery methods, off-target and nontarget effects, and potential development of resistance in insect populations.
英文关键词	dsRNA delivery RNAi efficiency RNAi pathway systemic RNAi pest management
类型	Review ; Book Chapter
语种	英语
国家	USA
收录类别	SCI-E
WOS记录号	WOS:000507471600015
WOS关键词	DOUBLE-STRANDED-RNA ; COLORADO POTATO BEETLE ; WESTERN CORN-ROOTWORM ; SYSTEMIC RNAI ; DESERT LOCUST ; LEPTINOTARSA-DECEMLINEATA ; GENETIC INTERFERENCE ; DSRNA DEGRADATION ; MIGRATORY LOCUST ; CROP PROTECTION
WOS类目	Entomology
WOS研究方向	Entomology
EI主题词	2020-01-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/312181
作者单位	1.Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA; 2.Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA
推荐引用方式 GB/T 7714	Zhu, Kun Yan,Palli, Subba Reddy. Mechanisms, Applications, and Challenges of Insect RNA Interference[J],2020,65:293-311.
APA	Zhu, Kun Yan,&Palli, Subba Reddy.(2020).Mechanisms, Applications, and Challenges of Insect RNA Interference.ANNUAL REVIEW OF ENTOMOLOGY, VOL 65,65,293-311.
MLA	Zhu, Kun Yan,et al."Mechanisms, Applications, and Challenges of Insect RNA Interference".ANNUAL REVIEW OF ENTOMOLOGY, VOL 65 65(2020):293-311.