干旱区生态环境知识资源中心(Arid): Higher expression levels of aquaporin (AQP)1 and AQP5 in the lungs of arid-desert living Lepus yarkandensis

Arid

DOI	10.1111/jpn.13272
	Higher expression levels of aquaporin (AQP)1 and AQP5 in the lungs of arid-desert living Lepus yarkandensis
	Zhang, Jianping 1,2,3; Li, Shuwei 1,2; Liu, Jie 3; Li, Lexing 3; Deng, Fang 1; Baikeli, Buheliqihan 1; Li, Linrui 4; Ma, Xuanye 4; Liu, Guoquan 3,4
通讯作者	Zhang, Jianping ; Liu, Guoquan
来源期刊	JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
ISSN	0931-2439
EISSN	1439-0396
出版年	2020
卷号	104 期号:4 页码:1186-1195
英文摘要	Water transport across epithelial cells that line the airways and alveoli is a crucial component of lung physiology. Aquaporins (AQPs) facilitate water transport across the air space-capillary barrier in the distal lung. However, the roles of lung AQPs in desert animal adaptation to dry airstream environments are still unclear. A hare (Lepus yarkandensis) only lives in the Tarim Basin, and its living environment is an arid climate with rare precipitation. We studied cellular localization and expression levels of AQP1, AQP3, AQP4 and AQP5 in L. yarkandensis lungs by immunohistochemistry, quantitative real-time polymerase chain reaction and Western blot. The lung of rabbits (Oryctolagus cuniculus) that inhabit in mesic environment was similarly studied. Obtained results in two species of animals were compared to investigate whether AQPs in the lung altered expression in the animal living in arid region. AQP1 was localized to the endothelial cells in capillaries and venules surrounding terminal bronchioles and alveoli. AQP5 was localized to the ciliated columnar cells in terminal bronchioles and the alveolar type I cells in the alveolus. Quantitative real-time PCR analysis showed higher AQP1 and AQP5 mRNA levels in L. yarkandensis compared to O. cuniculus. Similar results were obtained by Western blot. These results revealed that the higher expression levels of AQP1 and AQP5 played a significant role in water transport in the lungs of arid-desert living L. yarkandensis and might accelerate water transport from capillary compartments to the airspace.
英文关键词	AQP1 AQP5 arid-desert living Lepus yarkandensis lungs
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000502041800001
WOS关键词	WATER PERMEABILITY ; MOUSE LUNG ; EDEMA CLEARANCE ; FLUID TRANSPORT ; CHANNELS ; PROGRESS ; BARRIER ; TISSUES ; AIRWAYS
WOS类目	Agriculture, Dairy & Animal Science ; Veterinary Sciences
WOS研究方向	Agriculture ; Veterinary Sciences
EI主题词	2019-12-11
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/311688
作者单位	1.Tarim Univ, Coll Life Sci, Alar 843300, Peoples R China; 2.Tarim Univ, Key Lab Biol Resources Protect & Utilizat Tarim B, Alar, Peoples R China; 3.Bengbu Med Coll, Dept Biochem & Mol Biol, Sch Lab Med, Anhui Prov Key Lab Translat Canc Res, Bengbu, Peoples R China; 4.Huazhong Agr Univ, Dept Basic Vet Med, Key Lab Swine Genet & Breeding & Agr Anim Breedin, Coll Anim Sci & Vet Med, Wuhan, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jianping,Li, Shuwei,Liu, Jie,et al. Higher expression levels of aquaporin (AQP)1 and AQP5 in the lungs of arid-desert living Lepus yarkandensis[J],2020,104(4):1186-1195.
APA	Zhang, Jianping.,Li, Shuwei.,Liu, Jie.,Li, Lexing.,Deng, Fang.,...&Liu, Guoquan.(2020).Higher expression levels of aquaporin (AQP)1 and AQP5 in the lungs of arid-desert living Lepus yarkandensis.JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION,104(4),1186-1195.
MLA	Zhang, Jianping,et al."Higher expression levels of aquaporin (AQP)1 and AQP5 in the lungs of arid-desert living Lepus yarkandensis".JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION 104.4(2020):1186-1195.