干旱区生态环境知识资源中心(Arid): Phylogenetic characteristics of novel Bacillus weihenstephanensis and Pseudomonas sp. to desert locust, Schistocerca gregaria Forsk(a)over-circlel (Orthoptera: Acrididae)

Arid

DOI	10.1186/s41938-019-0188-7
	Phylogenetic characteristics of novel Bacillus weihenstephanensis and Pseudomonas sp. to desert locust, Schistocerca gregaria Forsk(a)over-circlel (Orthoptera: Acrididae)
	Mashtoly, Tamer A.1; El-Zemaity, Mohamed S.1; Abolmaaty, Assem 2; Abdelatef, Gamal M.3; Marzouk, Ashraf A.4; Reda, Mohamed 3
通讯作者	Mashtoly, Tamer A.
来源期刊	EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL
ISSN	1110-1768
EISSN	2536-9342
出版年	2019
卷号	29 期号:1
英文摘要	Thirty bacterial isolates were isolated from the gut contents of diseased/dead locust. Their pathogenicity was tested against 4th instar nymphs of desert locust, Schistocerca gregaria Forskal (Orthoptera: Acrididae). Two isolates, designated DL2 and DL6, out of thirty showed the highest insecticidal activities against locust nymphs in preliminary bracketing. They were bioassayed via leaf dip and per os techniques and toxicity was determined using SAS program. The insecticidal activity of DL6 was more than DL2, whereas LC50's values of 35 x 10(6) and 13 x 10(6) cfu's/ml were determined for DL2 and DL6, respectively, after 48 h of leaf-dip treatment. However, LD50's value of 53 x 10(6) and 26 x 10(6) cfu's/ml was determined for DL2 and DL6, respectively, after 24 h of per os treatment. The relative potencies of DL6 to DL2 were (2.6 and 2.03) folds in leaf-dip and per os treatments, respectively. Biochemical characterization was conducted, using GEN III MicroPlate (TM) Biolog identification system and confirmed with molecular identification via 16S rDNA gene sequencing. Nucleotide sequencing of each was submitted to a gene bank and an accession number was generated for each isolate. Obtained bacterial strains DL2 and DL6 were identified as Bacillus weihenstephanensis (KY630645) and Pseudomonas sp. (KY630649), with a similarity of 100 and 75% to B. weihenstephanensis strain PHCDB9 (NR_024697) and Pseudomonas sp. strain DSM11821 (KF417541), respectively. The tested strains proved their potential to be bio-pesticide agents involved in controlling desert locust nymphs.
英文关键词	Desert locust Entomopathogenic bacteria Microbial control Microbial identification 16S rRNA genes sequencing Biolog identification system Bio-pesticides
类型	Article
语种	英语
国家	Egypt
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000511117900001
WOS关键词	BACTERIA ; COLEOPTERA ; CEREUS ; TOXICITY ; PATHOGENICITY ; LEPIDOPTERA ; BIOLOG ; LARVAE ; IDENTIFICATION ; INFECTION
WOS类目	Entomology
WOS研究方向	Entomology
EI主题词	2019-12-09
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/311639
作者单位	1.Ain Shams Univ, Fac Agr, Dept Plant Protect, POB 68 Hadayek Shoubra, Cairo 11241, Egypt; 2.Ain Shams Univ, Fac Agr, Dept Food Sci, Cairo, Egypt; 3.Agr Res Ctr, Plant Protect Res Inst, Locust & Grasshoppers Res Dept, Cairo, Egypt; 4.Ain Shams Univ, Fac Agr, Microbiol Resources Ctr MIRCEN, Cairo, Egypt
推荐引用方式 GB/T 7714	Mashtoly, Tamer A.,El-Zemaity, Mohamed S.,Abolmaaty, Assem,et al. Phylogenetic characteristics of novel Bacillus weihenstephanensis and Pseudomonas sp. to desert locust, Schistocerca gregaria Forsk(a)over-circlel (Orthoptera: Acrididae)[J],2019,29(1).
APA	Mashtoly, Tamer A.,El-Zemaity, Mohamed S.,Abolmaaty, Assem,Abdelatef, Gamal M.,Marzouk, Ashraf A.,&Reda, Mohamed.(2019).Phylogenetic characteristics of novel Bacillus weihenstephanensis and Pseudomonas sp. to desert locust, Schistocerca gregaria Forsk(a)over-circlel (Orthoptera: Acrididae).EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL,29(1).
MLA	Mashtoly, Tamer A.,et al."Phylogenetic characteristics of novel Bacillus weihenstephanensis and Pseudomonas sp. to desert locust, Schistocerca gregaria Forsk(a)over-circlel (Orthoptera: Acrididae)".EGYPTIAN JOURNAL OF BIOLOGICAL PEST CONTROL 29.1(2019).