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Thirty-six species of bivalves, belonging to 22 genera, representative of 16 orders, are systematically described from five upper Campanian-lower Eocene sections in the Kharga Oasis. Stratigraphically, 15 species were recorded from the Palaeocene, 8 from the lower Eocene, 8 from the Maastrichtian, 3 from the Campanian, 1 from the Campanian-Maastrichtian and 1 from the Maastrichtian-Palaeocene. The paleobiogeographic distribution of the Campanian-Maastrichtian bivalves reveals high degree of marine connection between the South Tethyan province and the West Africa, Middle East, southern Europe, South America and India provinces. Consequently, the Campanian-Maastrichtian bivalves have a strong southern Tethyan affinity. The early Palaeogene bivalves are markedly endemic to the Egyptian territory, which can argued to the isolation in marine environment by the localised tectonic events and/or the pronounced climatic changes. Accordingly, the palaeobiogeographic distribution of the studied bivalves was wider in the Late Cretaceous (Campanian-Maastrichtian) than in the early Palaeogene (Palaeocene-early Eocene).