干旱区生态环境知识资源中心(Arid): Various Inc-type plasmids and lineages of Escherichia coli and Klebsiella pneumoniae spreading bla(CTX-M-15), bla(CTX-M-1) and mcr-1 genes in camels in Tunisia

Arid

DOI	10.1016/j.jgar.2019.05.007
	Various Inc-type plasmids and lineages of Escherichia coli and Klebsiella pneumoniae spreading bla(CTX-M-15), bla(CTX-M-1) and mcr-1 genes in camels in Tunisia
	Saidani, Mariem 1,2; Messadi, Lilia 1; Mefteh, Jomaa 1; Chaouechi, Amira 1; Soudani, Alya 1; Selmi, Rachid 1; Daaloul-Jedidi, Monia 1; Ben Chehida, Faten 1; Mamlouk, Aymen 1; Jemli, Mohamed Habib 3; Madec, Jean-Yves 4; Haenni, Marisa 4
通讯作者	Haenni, Marisa
来源期刊	JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
ISSN	2213-7165
EISSN	2213-7173
出版年	2019
卷号	19 页码:280-283
英文摘要	Objectives: Resistance to extended-spectrum cephalosporins, fluoroquinolones and colistin is under constant scrutiny in food-producing animals worldwide. However, little is known about camels, which provide milk and meat for human consumption, and are attractions for tourists to ride in arid regions. This study assessed the role of camels as potential reservoirs of these resistance determinants. Methods: Faecal swabs were collected from 232 camels in Tunisia between April 2016 and July 2018. Enterobacteriaceae were detected on MacConkey agar and extended-spectrum beta-lactamase (ESBL)-producers on the same medium supplemented with cefotaxime. Antimicrobial resistance was assessed by disc diffusion, and ESBL-producing isolates were further characterised by phylogrouping (for Escherichia coli, E. coli) and multilocus sequence typing. Genetic support of the bla(ESBL) and mcr-1 genes was identified by plasmid-typing and Southern blot. Results: E. coli were identified in 163 of 232 (70.3%) and Klebsiella pneumoniae (K. pneumoniae) in 16 of 232 (6.9%) of the dominant flora. Three E. coli and one K. pneumoniae (1.3% and 0.4%, respectively) were found on cefotaxime-enriched media. One K. pneumoniae and one E. coli from a tourist farm harboured the bla(CTX-M-15) gene on an IncY plasmid, while the two E. coli from the butchery sector displayed the bla(CTX-M-15) gene on an IncI1 plasmid and colocalisation of the bla(CTX-M-1) and mcr-1 genes on an IncHI2 plasmid. Conclusions: This study reported ESBL-producing Enterobacteriaceae in Tunisian camels from both tourist and meat-producing sectors. This was the first description of the mcr-1 gene in a meat-producing camel. Although not alarming, this context needs specific attention to avoid camels becoming a bigger reservoir for multidrug-resistant Enterobacteriaceae. (C) 2019 International Society for Antimicrobial Chemotherapy. Published by Elsevier Ltd. All rights reserved.
英文关键词	Camel mcr-1 CTX-M Escherichia coli Klebsiella pneumoniae Tunisia
类型	Article
语种	英语
国家	Tunisia ; France
收录类别	SCI-E
WOS记录号	WOS:000501565800054
WOS关键词	RESISTANCE ; ANIMALS ; STRAINS ; PETS
WOS类目	Infectious Diseases ; Pharmacology & Pharmacy
WOS研究方向	Infectious Diseases ; Pharmacology & Pharmacy
EI主题词	2019-12-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/311276
作者单位	1.Univ La Manouba, Ecole Natl Med Vet, Serv Microbiol & Immunol, Sidi Thabet, Tunisia; 2.Univ Carthage, Inst Natl Agron Tunisie, Tunis, Tunisia; 3.Univ La Manouba, Ecole Natl Med Vet, Serv Parasitol, Sidi Thabet, Tunisia; 4.Univ Lyon, ANSES, Unite Antibioresistance & Virulence Bacteriennes, Site Lyon,31 Ave Tony Garnier, F-69364 Lyon, France
推荐引用方式 GB/T 7714	Saidani, Mariem,Messadi, Lilia,Mefteh, Jomaa,et al. Various Inc-type plasmids and lineages of Escherichia coli and Klebsiella pneumoniae spreading bla(CTX-M-15), bla(CTX-M-1) and mcr-1 genes in camels in Tunisia[J],2019,19:280-283.
APA	Saidani, Mariem.,Messadi, Lilia.,Mefteh, Jomaa.,Chaouechi, Amira.,Soudani, Alya.,...&Haenni, Marisa.(2019).Various Inc-type plasmids and lineages of Escherichia coli and Klebsiella pneumoniae spreading bla(CTX-M-15), bla(CTX-M-1) and mcr-1 genes in camels in Tunisia.JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE,19,280-283.
MLA	Saidani, Mariem,et al."Various Inc-type plasmids and lineages of Escherichia coli and Klebsiella pneumoniae spreading bla(CTX-M-15), bla(CTX-M-1) and mcr-1 genes in camels in Tunisia".JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE 19(2019):280-283.