干旱区生态环境知识资源中心(Arid): New genus-specific primers for PCR identification of Rubrobacter strains

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DOI	10.1007/s10482-019-01314-3
	New genus-specific primers for PCR identification of Rubrobacter strains
	Franco Castro, Jean 1,2; Nouioui, Imen 1; Asenjo, Juan A.2; Andrews, Barbara 2; Bull, Alan T.3; Goodfellow, Michael 1
通讯作者	Goodfellow, Michael
来源期刊	ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
ISSN	0003-6072
EISSN	1572-9699
出版年	2019
卷号	112 期号:12 页码:1863-1874
英文摘要	A set of oligonucleotide primers, Rubro223f and Rubro454r, were found to amplify a 267 nucleotide sequence of 16S rRNA genes of Rubrobacter type strains. The primers distinguished members of this genus from other deeply-rooted actinobacterial lineages corresponding to the genera Conexibacter, Gaiella, Parviterribacter, Patulibacter, Solirubrobacter and Thermoleophilum of the class Thermoleophilia. Amplification of DNA bands of about 267 nucleotides were generated from environmental DNA extracted from soil samples taken from two locations in the Atacama Desert. Sequencing of a DNA library prepared from the bands showed that all of the clones fell within the evolutionary radiation occupied by the genus Rubrobacter. Most of the clones were assigned to two lineages that were well separated from phyletic lines composed of Rubrobacter type strains. It can be concluded that primers Rubro223f and Rubro454r are specific for the genus Rubrobacter and can be used to detect the presence and abundance of members of this genus in the Atacama Desert and other biomes.
英文关键词	Actinobacteria Rubrobacter Atacama desert Taxonomy Genus-specific primers
类型	Article
语种	英语
国家	England ; Chile
开放获取类型	Green Accepted, Green Published, hybrid
收录类别	SCI-E
WOS记录号	WOS:000494890500015
WOS关键词	16S RIBOSOMAL-RNA ; SP-NOV. ; BACTERIAL DIVERSITY ; EMENDED DESCRIPTION ; ARTHROBACTER-RADIOTOLERANS ; CLASS ACTINOBACTERIA ; SOIL BACTERIA ; ARID SOILS ; FAM. NOV ; PROPOSAL
WOS类目	Microbiology
WOS研究方向	Microbiology
EI主题词	2019-12-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/311152
作者单位	1.Newcastle Univ, Sch Nat & Environm Sci, Ridley Bldg, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England; 2.Univ Chile, Ctr Biotechnol & Bioengn CeBiB, Dept Chem Engn Biotechnol & Mat, Beauchef 851, Santiago, Chile; 3.Univ Kent, Sch Biosci, Canterbury CT2 1NJ, Kent, England
推荐引用方式 GB/T 7714	Franco Castro, Jean,Nouioui, Imen,Asenjo, Juan A.,et al. New genus-specific primers for PCR identification of Rubrobacter strains[J],2019,112(12):1863-1874.
APA	Franco Castro, Jean,Nouioui, Imen,Asenjo, Juan A.,Andrews, Barbara,Bull, Alan T.,&Goodfellow, Michael.(2019).New genus-specific primers for PCR identification of Rubrobacter strains.ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY,112(12),1863-1874.
MLA	Franco Castro, Jean,et al."New genus-specific primers for PCR identification of Rubrobacter strains".ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY 112.12(2019):1863-1874.