干旱区生态环境知识资源中心(Arid): Associations between body condition score at parturition and microRNA profile in colostrum of dairy cows as evaluated by paired mapping programs

Arid

DOI	10.3168/jds.2019-16675
	Associations between body condition score at parturition and microRNA profile in colostrum of dairy cows as evaluated by paired mapping programs
	Ylioja, C. M.; Rolf, M. M.; Mamedova, L. K.; Bradford, B. J.
通讯作者	Bradford, B. J.
来源期刊	JOURNAL OF DAIRY SCIENCE
ISSN	0022-0302
EISSN	1525-3198
出版年	2019
卷号	102 期号:12 页码:11609-11621
英文摘要	MicroRNA (miRNA) are abundant in milk, and likely have regulatory activity involving lactation and immunity. The objective of this study was to determine the miRNA profile in colostrum of overconditioned cows compared with cows of more moderate body condition score (BCS) at calving. Multiparous cows with either high (>= 4.0 on a scale of 1 to 5; n = 7) or moderate BCS (2.75 to 3.50; n = 9) in the week before parturition were selected from a commercial dairy herd. Blood and colostrum were sampled within 24 h after calving. Blood serurri was analyzed for free fatty acid (FFA) concentration. MicroRNA was isolated from colostrum samples after removing milk fat and cells. MicroRNA were sequenced, and reads were mapped to the bovine genome and to the existing database of miRNA at miR-Base.org . Two programs, Oasis 2.0 and miRDeep2, were employed in parallel for read alignment, and analysis of miRNA count data was performed using DESeq2. Identification of differentially expressed miRNA from DESeq2 was not affected by the differences in miRNA detected by the 2 mapping programs. Most abundant miRNA included miR-30a, miR-148a, miR-181a, let-7f, miR-26a, rniR-21, miR-22, and miR-92a. Large-scale shifts in miRNA profile were not observed; however, colostrum of cows with high BCS contained less miR-486, which has been linked with altered glucose metabolism. Colostrum from cows with elevated serum FFA contained less miR-885, which may be connected to hepatic function during the transition period. Potential functions of abundant miRNA suggest involvement in development arid maintenance of cellular function in the mammary gland, with the additional possibility of influencing neonatal tissue and immune system development.
英文关键词	RNA silencing neonate transition cow immune function bioinformatics
类型	Article
语种	英语
国家	USA
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:000496786100083
WOS关键词	BOVINE-MILK EXOSOMES ; DICER EXPRESSION ; MESSENGER-RNA ; LACTATION ; CELLS ; MIRNAS ; GENES ; PROLIFERATION ; BIOGENESIS ; ACTIVATION
WOS类目	Agriculture, Dairy & Animal Science ; Food Science & Technology
WOS研究方向	Agriculture ; Food Science & Technology
EI主题词	2019-12-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/311144
作者单位	Kansas State Univ, Dept Anim Sci & Ind, Manhattan, KS 66506 USA
推荐引用方式 GB/T 7714	Ylioja, C. M.,Rolf, M. M.,Mamedova, L. K.,et al. Associations between body condition score at parturition and microRNA profile in colostrum of dairy cows as evaluated by paired mapping programs[J],2019,102(12):11609-11621.
APA	Ylioja, C. M.,Rolf, M. M.,Mamedova, L. K.,&Bradford, B. J..(2019).Associations between body condition score at parturition and microRNA profile in colostrum of dairy cows as evaluated by paired mapping programs.JOURNAL OF DAIRY SCIENCE,102(12),11609-11621.
MLA	Ylioja, C. M.,et al."Associations between body condition score at parturition and microRNA profile in colostrum of dairy cows as evaluated by paired mapping programs".JOURNAL OF DAIRY SCIENCE 102.12(2019):11609-11621.