干旱区生态环境知识资源中心(Arid): Genome analysis provides insights into crude oil degradation and biosurfactant production by extremely halotolerant Halomonas desertis G11 isolated from Chott El-Djerid salt-lake in Tunisian desert

Arid

DOI	10.1016/j.ygeno.2018.12.003
	Genome analysis provides insights into crude oil degradation and biosurfactant production by extremely halotolerant Halomonas desertis G11 isolated from Chott El-Djerid salt-lake in Tunisian desert
	Neifar, Mohamed 1; Chouchane, Habib 1; Najjari, Afef 1; El Hidri, Darine 1; Mahjoubi, Mouna 1; Ghedira, Kais 2; Naili, Fatma 1; Soufi, Leila 1; Raddadi, Noura 3; Sghaier, Haitham 4,5; Ouzari, Hadda I.6; Masmoudi, Ahmed Salaheddine 1; Cherif, Ameur 1
通讯作者	Neifar, Mohamed ; Cherif, Ameur
来源期刊	GENOMICS
ISSN	0888-7543
EISSN	1089-8646
出版年	2019
卷号	111 期号:6 页码:1802-1814
英文摘要	Here, we report the genomic features and the bioremediation potential of Halomonas desertis G11, a new halophilic species which uses crude oil as a carbon and energy source and displays intrinsic resistance to salt stress conditions (optimum growth at 10% NaCl). G11 genome (3.96 Mb) had a mean GC content of 57.82%, 3622 coding sequences, 480 subsystems and 64 RNA genes. Annotation predicted 38 genes involved in osmotic stress including the biosynthesis of osmoprotectants glycine-betaine, ectoine and osmoregulated periplasmic glucans. Genome analysis revealed also the versatility of the strain for emulsifying crude oil and metabolizing hydrocarbons. The ability of G11 to degrade crude oil components and to secrete a glycolipid biosurfactant with satisfying properties was experimentally confirmed and validated. Our results help to explain the exceptional capacity of G11 to survive at extreme desertic conditions, and highlight the metabolic features of this organism that has biotechnological and ecological potentialities.
英文关键词	Genome sequencing Halomonas desertis Crude oil biodegradation Biosurfactant production Osmoadaptation Metabolic pathways
类型	Article
语种	英语
国家	Tunisia ; Italy
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:000495876600070
WOS关键词	PHYLOGENETIC ANALYSIS ; HALOPHILIC BACTERIUM ; SP NOV. ; ANNOTATION ; BIODEGRADATION ; SEQUENCE ; RHAMNOLIPIDS ; DATABASE ; ECTOINE ; SYSTEM
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
EI主题词	2019-12-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/311105
作者单位	1.Univ Manouba, BVBGR LR11ES31, ISBST, Biotechpole Sidi Thabet, Ariana 2020, Tunisia; 2.Pasteur Inst Tunis, Lab BioInformat BioMath & BioStat BIMS LR16IPT09, 13 Pl Pasteur,BP 74, Tunis 1002, Tunisia; 3.Univ Bologna, Dept Civil Chem Environm & Mat Engn DICAM, Bologna, Italy; 4.Natl Ctr Nucl Sci & Technol CNSTN, Lab Energy & Matter Dev Nucl Sci LR16CNSTN02, Sidi Thabet Technopk, Sidi Thabet 2020, Tunisia; 5.Lab Biotechnol & Biogeo Resources Valorizat LR11E, Associated Lab Biotechnol & Nucl Technol LR16CNST, Sidi Thabet Technopk, Sidi Thabet 2020, Tunisia; 6.Univ Tunis El Manar, Fac Sci Tunis, Lab Microorganisms & Act Biomol, Tunis 2092, Tunisia
推荐引用方式 GB/T 7714	Neifar, Mohamed,Chouchane, Habib,Najjari, Afef,et al. Genome analysis provides insights into crude oil degradation and biosurfactant production by extremely halotolerant Halomonas desertis G11 isolated from Chott El-Djerid salt-lake in Tunisian desert[J],2019,111(6):1802-1814.
APA	Neifar, Mohamed.,Chouchane, Habib.,Najjari, Afef.,El Hidri, Darine.,Mahjoubi, Mouna.,...&Cherif, Ameur.(2019).Genome analysis provides insights into crude oil degradation and biosurfactant production by extremely halotolerant Halomonas desertis G11 isolated from Chott El-Djerid salt-lake in Tunisian desert.GENOMICS,111(6),1802-1814.
MLA	Neifar, Mohamed,et al."Genome analysis provides insights into crude oil degradation and biosurfactant production by extremely halotolerant Halomonas desertis G11 isolated from Chott El-Djerid salt-lake in Tunisian desert".GENOMICS 111.6(2019):1802-1814.