干旱区生态环境知识资源中心(Arid): De novo transcriptome sequencing and identification of genes related to salt and PEG stress in Tetraena mongolica Maxim

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DOI	10.1007/s00468-019-01886-7
	De novo transcriptome sequencing and identification of genes related to salt and PEG stress in Tetraena mongolica Maxim
	Chen, Ningmei; Feng, Jinchao; Song, Buerbatu; Tang, Shuai; He, Junqing; Zhou, Yijun; Shi, Sha; Xu, Xiaojing
通讯作者	Xu, Xiaojing
来源期刊	TREES-STRUCTURE AND FUNCTION
ISSN	0931-1890
EISSN	1432-2285
出版年	2019
卷号	33 期号:6 页码:1639-1656
英文摘要	Key message The first transcriptome analyses of Tetraena mongolica provided 224,618 Unigenes and identified the genes that were differentially expressed under salt and drought conditions, giving some insights into the unique adaptive capacity of this relic plant. Tetraena mongolica Maxim, the only member of the Tetraena genus in the Zygophyllaceae, is endemic to the northwest of China. As one of the relic shrubs of the Paleo-Mediterranean flora, T. mongolica plays a key role in preserving the local ecological environment. To investigate its good adaptability in desert, we studied the transcriptome of T. mongolica under NaCl and PEG6000 stresses. Three libraries were constructed from a mixture of seedlings and mature plants of T. mongolica, and the de novo transcriptome was sequenced using an Illumina HiSeq 4000. Approximately 218.15 million clean reads were assembled de novo into 383,612 transcripts, and 116,027 All-Unigenes were identified. By aligning All-Unigene sequences against the NR database, we found that most of the All-Unigenes had very low matches with the sequences from other plants. A total of 21,112 SSRs and 6 types of SNP variants were identified. The RNA-Seq data revealed 60 transcription factor (TF) families with 3163 genes in total. Six libraries were constructed from the separate control or stressed seedlings, and the transcriptome was sequenced using a BGISEQ-500 platform. There are 1105 and 1383 differentially expressed genes (DEGs) with 42 and 54 TFs under NaCl and PEG6000 treatment, respectively. The genes that were putatively involved in salt and osmotic stresses were searched and analyzed. Quantitative reverse transcription PCR (qRT-PCR) showed that DEG expression profiles were consistent with those from RNA-seq (RNA sequencing). Overall, this study provides new insights into the molecular mechanisms that control salt and drought stress responses in T. mongolica.
英文关键词	Tetraena mongolica Transcriptome sequencing Unigenes Differentially expressed gene Stress response
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000494830600010
WOS关键词	RNA-SEQ ; FUNCTIONAL-ANALYSIS ; SALINITY TOLERANCE ; REGULATORY NETWORK ; RESPONSIVE GENES ; RELIC SHRUBS ; DROUGHT ; EXPRESSION ; WATER ; MARKERS
WOS类目	Forestry
WOS研究方向	Forestry
EI主题词	2019-12-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/311103
作者单位	Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Chen, Ningmei,Feng, Jinchao,Song, Buerbatu,et al. De novo transcriptome sequencing and identification of genes related to salt and PEG stress in Tetraena mongolica Maxim[J],2019,33(6):1639-1656.
APA	Chen, Ningmei.,Feng, Jinchao.,Song, Buerbatu.,Tang, Shuai.,He, Junqing.,...&Xu, Xiaojing.(2019).De novo transcriptome sequencing and identification of genes related to salt and PEG stress in Tetraena mongolica Maxim.TREES-STRUCTURE AND FUNCTION,33(6),1639-1656.
MLA	Chen, Ningmei,et al."De novo transcriptome sequencing and identification of genes related to salt and PEG stress in Tetraena mongolica Maxim".TREES-STRUCTURE AND FUNCTION 33.6(2019):1639-1656.