干旱区生态环境知识资源中心(Arid): Regulatory changes in TaSNAC8-6A are associated with drought tolerance in wheat seedlings

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DOI	10.1111/pbi.13277
	Regulatory changes in TaSNAC8-6A are associated with drought tolerance in wheat seedlings
	Mao, Hude; Li, Shumin; Wang, Zhongxue; Cheng, Xinxiu; Li, Fangfang; Mei, Fangming; Chen, Nan; Kang, Zhensheng
通讯作者	Mao, Hude
来源期刊	PLANT BIOTECHNOLOGY JOURNAL
ISSN	1467-7644
EISSN	1467-7652
出版年	2020
卷号	18 期号:4 页码:1078-1092
英文摘要	Wheat is a staple crop produced in arid and semi-arid areas worldwide, and its production is frequently compromised by water scarcity. Thus, increased drought tolerance is a priority objective for wheat breeding programmes, and among their targets, the NAC transcription factors have been demonstrated to contribute to plant drought response. However, natural sequence variations in these genes have been largely unexplored for their potential roles in drought tolerance. Here, we conducted a candidate gene association analysis of the stress-responsive NAC gene subfamily in a wheat panel consisting of 700 varieties collected worldwide. We identified a drought responsive gene, TaSNAC8-6A, that is tightly associated with drought tolerance in wheat seedlings. Further analysis found that a favourable allele TaSNAC8-6A(In-313), carrying an insertion in the ABRE promoter motif, is targeted by TaABFs and confers enhanced drought-inducible expression of TaSNAC8-6A in drought-tolerant genotypes. Transgenic wheat and Arabidopsis TaSNAC8-6A overexpression lines exhibited enhanced drought tolerance through induction of auxin- and drought-response pathways, confirmed by transcriptomic analysis, that stimulated lateral root development, subsequently improving water-use efficiency. Taken together, our findings reveal that natural variation in TaSNAC8-6A and specifically the TaSNAC8-6A(In-313) allele strongly contribute to wheat drought tolerance and thus represent a valuable genetic resource for improvement of drought-tolerant germplasm for wheat production.
英文关键词	wheat NAC transcription factor TaSNAC8-6A natural variation lateral root development drought stress
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000497118300001
WOS关键词	NAC TRANSCRIPTION FACTOR ; GENOME-WIDE ASSOCIATION ; STRESS-RESPONSE ; GRAIN-YIELD ; OSMOTIC-STRESS ; GENE ; ARABIDOPSIS ; RICE ; RESISTANCE ; SENESCENCE
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
EI主题词	2019-11-19
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310931
作者单位	Northwest A&F Univ, Coll Plant Protect, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Mao, Hude,Li, Shumin,Wang, Zhongxue,et al. Regulatory changes in TaSNAC8-6A are associated with drought tolerance in wheat seedlings[J]. 西北农林科技大学,2020,18(4):1078-1092.
APA	Mao, Hude.,Li, Shumin.,Wang, Zhongxue.,Cheng, Xinxiu.,Li, Fangfang.,...&Kang, Zhensheng.(2020).Regulatory changes in TaSNAC8-6A are associated with drought tolerance in wheat seedlings.PLANT BIOTECHNOLOGY JOURNAL,18(4),1078-1092.
MLA	Mao, Hude,et al."Regulatory changes in TaSNAC8-6A are associated with drought tolerance in wheat seedlings".PLANT BIOTECHNOLOGY JOURNAL 18.4(2020):1078-1092.