干旱区生态环境知识资源中心(Arid): Root-Colonizing Endophytic Fungi of the Dominant Grass Stipa krylovii From a Mongolian Steppe Grassland

Arid

DOI	10.3389/fmicb.2019.02565
	Root-Colonizing Endophytic Fungi of the Dominant Grass Stipa krylovii From a Mongolian Steppe Grassland
	Knapp, Daniel G.1; Imrefi, Ildiko 1; Boldpurev, Enkhtuul 1; Csikos, Sandor 1; Akhmetova, Galiya 1; Berek-Nagy, Peter Janos 1; Otgonsuren, Burenjargal 2; Kovacs, Gabor M.1
通讯作者	Knapp, Daniel G. ; Kovacs, Gabor M.
来源期刊	FRONTIERS IN MICROBIOLOGY
EISSN	1664-302X
出版年	2019
卷号	10
英文摘要	In several terrestrial ecosystems such as grasslands, plants live together with various root-colonizing dark septate endophytes (DSEs), fungi that are relatively frequent colonizers of healthy belowground tissues of plants in these environments. They are important members of the plant microbiota and may have various effects on plant survival under different stress conditions; however, their general functions in relation to plants and the greater ecosystem remain elusive. Although an increasing number of studies has been published focusing on DSEs in Asian grasslands, our knowledge is limited. Especially in Mongolia, where the steppe region represents a significant area, information is not available on these root colonizers. In this study, we aimed to characterize DSEs of a common dominant gramineous plant species, Stipa krylovii in a semiarid grassland of Mongolia. Root samples were collected in a natural steppe and were processed for isolation of fungal endophytes. For molecular identification of the isolates, the internal transcribed spacer (ITS) region of the nrDNA was obtained for all the isolates investigated; furthermore, the partial translation elongation factor 1-alpha (TEF) gene and large subunit (LSU) and small subunit (SSU) of rDNA were also amplified and sequenced in case of representative isolates. In vitro tests were used to examine the rough symbiotic nature of the fungi, and root colonization was visualized. A majority of the 135 isolates examined in detail was found to belong to several orders of Ascomycota (110 isolates) and some to Basidiomycota (25 isolates). A significant number of the isolates represented presumably novel taxa, and dominant similarities of the lineages have been found with relatively frequent and known grass root endophytes of semiarid areas in other geographic regions. These endophytes included Periconia macrospinosa, Microdochium bolley, and Darksidea, the genus of which comprised one fourth of the isolates. We found numerous lineages, which have been detected not only from Asian steppe ecosystems, but also from prairies in North America and sandy grasslands in Europe. Therefore, our results strengthen the hypothesized worldwide presence of a common and dominant core group of a DSE community in arid and semiarid grasslands.
英文关键词	Asia diversity phylogeny Pleosporales root-associated fungi
类型	Article
语种	英语
国家	Hungary ; Mongolia
开放获取类型	gold, Green Published, Green Accepted
收录类别	SCI-E
WOS记录号	WOS:000499217400001
WOS关键词	DARK SEPTATE ENDOPHYTES ; INNER-MONGOLIA ; ARBUSCULAR MYCORRHIZAL ; SPATIAL DYNAMICS ; RIBOSOMAL DNA ; DIVERSITY ; CHINA ; IDENTIFICATION ; COLONIZATION ; RANGELAND
WOS类目	Microbiology
WOS研究方向	Microbiology
EI主题词	2019-11-12
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310839
作者单位	1.Eotvos Lorand Univ, Inst Biol, Dept Plant Anat, Budapest, Hungary; 2.Mongolian Univ Life Sci, Dept Ecol, Ulaanbaatar, Mongolia
推荐引用方式 GB/T 7714	Knapp, Daniel G.,Imrefi, Ildiko,Boldpurev, Enkhtuul,et al. Root-Colonizing Endophytic Fungi of the Dominant Grass Stipa krylovii From a Mongolian Steppe Grassland[J],2019,10.
APA	Knapp, Daniel G..,Imrefi, Ildiko.,Boldpurev, Enkhtuul.,Csikos, Sandor.,Akhmetova, Galiya.,...&Kovacs, Gabor M..(2019).Root-Colonizing Endophytic Fungi of the Dominant Grass Stipa krylovii From a Mongolian Steppe Grassland.FRONTIERS IN MICROBIOLOGY,10.
MLA	Knapp, Daniel G.,et al."Root-Colonizing Endophytic Fungi of the Dominant Grass Stipa krylovii From a Mongolian Steppe Grassland".FRONTIERS IN MICROBIOLOGY 10(2019).