干旱区生态环境知识资源中心(Arid): Transcriptome responses to different herbivores reveal differences in defense strategies between populations of Eruca sativa

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DOI	10.1186/s12864-019-6217-9
	Transcriptome responses to different herbivores reveal differences in defense strategies between populations of Eruca sativa
	Ogran, Ariel; Faigenboim, Adi; Barazani, Oz
通讯作者	Barazani, Oz
来源期刊	BMC GENOMICS
ISSN	1471-2164
出版年	2019
卷号	20 期号:1
英文摘要	BackgroundIntraspecific variations among induced responses might lead to understanding of adaptive variations in defense strategies against insects. We employed RNA-Seq transcriptome screening to elucidate the molecular basis for phenotypic differences between two populations of Eruca sativa (Brassicaceae), in defense against larvae of the generalist and specialist insects, Spodoptera littoralis and Pieris brassicae, respectively. The E. sativa populations originated from desert and Mediterranean sites, where the plants grow in distinct habitats.ResultsResponses to elicitation of the plants' defenses against wounding and insect herbivory resulted in more upregulated transcripts in plants of the Mediterranean population than in those of the desert. PCA analysis differentiated between the two populations and between the elicitation treatments. Comprehensive analysis indicated that defense responses involved induction of the salicylic acid and jasmonic acid pathways in plants of the desert and Mediterranean populations, respectively. In general, the defense response involved upregulation of the aliphatic glucosinolates pathway in plants of the Mediterranean population, whereas herbivory caused downregulation of this pathway in desert plants. Further quantitative RT-PCR analysis indicated that defense response in the desert plants involved higher expression of nitrile-specifier protein (NSP) than in the Mediterranean plants, suggesting that in the desert plants glucosinolates breakdown products are directed to simple-nitriles rather than to the more toxic isothiocyanates. In addition, the defense response in plants of the desert population involved upregulation of flavonoid synthesis and sclerophylly.ConclusionsThe results indicated that differing defense responses in plants of the two populations are governed by different signaling cascades. We suggest that adaptive ecotypic differentiation in defense strategies could result from generalist and specialist herbivore pressures in the Mediterranean and desert populations, respectively. Moreover, the defense responses in plants of the desert habitat, which include upregulation of mechanical defenses, also could be associated with their dual role in defense against both biotic and abiotic stresses.
英文关键词	RNA Seq Jasmonic acid Salicylic acid Glucosinolates Generalist vs specialist insects
类型	Article
语种	英语
国家	Israel
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000497733700004
WOS关键词	ARABIDOPSIS-THALIANA ; GLUCOSINOLATE HYDROLYSIS ; PLANT-RESISTANCE ; GENE-EXPRESSION ; FITNESS COSTS ; SPECIALIST ; GENERALIST ; BIOCONDUCTOR ; MODULATION ; VOLATILES
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
EI主题词	2019-11-12
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310828
作者单位	Agr Res Org, Volcani Ctr, Inst Plant Sci, IL-7505101 Rishon Leziyyon, Israel
推荐引用方式 GB/T 7714	Ogran, Ariel,Faigenboim, Adi,Barazani, Oz. Transcriptome responses to different herbivores reveal differences in defense strategies between populations of Eruca sativa[J],2019,20(1).
APA	Ogran, Ariel,Faigenboim, Adi,&Barazani, Oz.(2019).Transcriptome responses to different herbivores reveal differences in defense strategies between populations of Eruca sativa.BMC GENOMICS,20(1).
MLA	Ogran, Ariel,et al."Transcriptome responses to different herbivores reveal differences in defense strategies between populations of Eruca sativa".BMC GENOMICS 20.1(2019).