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DOI	10.1186/s12864-019-6169-0
	Characterization and discovery of miRNA and miRNA targets from apomictic and sexual genotypes of Eragrostis curvula
	Garbus, Ingrid 1; Pablo Selva, Juan 1; Cielo Pasten, Maria 1; Martin Bellido, Andres 1; Carballo, Jose 1; Albertini, Emidio 2; Echenique, Viviana 1
通讯作者	Garbus, Ingrid
来源期刊	BMC GENOMICS
ISSN	1471-2164
出版年	2019
卷号	20 期号:1
英文摘要	Background Weeping lovegrass (Eragrostis curvula [Shrad.] Nees) is a perennial grass found in semi-arid regions that is well adapted for growth in sandy soils and drought conditions. E. curvula constitutes a polymorphic complex that includes cytotypes with different ploidy levels (from 2x to 8x), where most polyploids are facultative apomicts, although both sexual reproduction and full apomixis have been reported in this species. Apomixis is thought to be associated with silencing of the sexual pathway, which would involve epigenetic mechanisms. However, a correlation between small RNAs and apomixis has not yet been conclusively established. Results Aiming to contribute to the elucidation of their role in the expression of apomixis, we constructed small RNA libraries from sexual and apomictic E. curvula genotypes via Illumina technology, characterized the small RNA populations, and conducted differential expression analysis by comparing these small RNAs with the E. curvula reference transcriptome. We found that the expression of two genes is repressed in the sexual genotype, which is associated with specific microRNA expression. Conclusion Our results support the hypothesis that in E. curvula the expression of apomixis leads to sexual repression.
英文关键词	Eragrostis curvula Apomixis Small RNA libraries miRNAs miRNA target
类型	Article
语种	英语
国家	Argentina ; Italy
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000497733700005
WOS关键词	SEQUENCE-TAG ANALYSIS ; APOMIXIS ; EXPRESSION ; ANGIOSPERMS ; MICRORNAS ; IDENTIFICATION ; EVOLUTION ; INSIGHTS ; GENES
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
EI主题词	2019-11-12
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310827
作者单位	1.Consejo Nacl Invest Cient & Tecn, Ctr Recursos Nat Renovables Zona Semiarida CERZOS, Bahia Blanca, Buenos Aires, Argentina; 2.Univ Perugia, Dipartimento Sci Agr Alimentari & Ambientali, Perugia, Italy
推荐引用方式 GB/T 7714	Garbus, Ingrid,Pablo Selva, Juan,Cielo Pasten, Maria,et al. Characterization and discovery of miRNA and miRNA targets from apomictic and sexual genotypes of Eragrostis curvula[J],2019,20(1).
APA	Garbus, Ingrid.,Pablo Selva, Juan.,Cielo Pasten, Maria.,Martin Bellido, Andres.,Carballo, Jose.,...&Echenique, Viviana.(2019).Characterization and discovery of miRNA and miRNA targets from apomictic and sexual genotypes of Eragrostis curvula.BMC GENOMICS,20(1).
MLA	Garbus, Ingrid,et al."Characterization and discovery of miRNA and miRNA targets from apomictic and sexual genotypes of Eragrostis curvula".BMC GENOMICS 20.1(2019).