干旱区生态环境知识资源中心(Arid): Identification and characterisation of heat shock protein gene (HSP70) family and its expression in Agave sisalana under heat stress

Arid

DOI	10.1080/14620316.2019.1685412
	Identification and characterisation of heat shock protein gene (HSP70) family and its expression in Agave sisalana under heat stress
	Batcho, Anicet Agossa; Sarwar, Muhammad Bilal; Tariq, Leeza; Rashid, Bushra; Hassan, Sameera; Husnain, Tayyab
通讯作者	Rashid, Bushra
来源期刊	JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
ISSN	1462-0316
EISSN	2380-4084
出版年	2020
卷号	95 期号:4 页码:470-482
英文摘要	Heat shock Protein (HSP) expression is the plants' responses to high temperature. We analysed the HSP70 gene family in Agave sisalana transcriptome to understand its expression under heat stress. A total of 167 genes were identified as heat shock protein genes. Among them; 74, 39 and 25 belong to the HSP70, HSP20 HSP90 respectively and multiple sequence alignment of conserved domain proteins of AsHSP70 showed that they are highly conserved. Fifty-two AsHSPs were classified into three major classes according to their structural characteristics. Phylogenetic analysis indicated that orthologs from Agave deserti and Agave tequilana species were distributed among the three classes; which revealed the diverse expression of HSP70 gene family. In silico subcellular localisation of AsHSP, suggested that its interaction is quite diverse in target cells and active signalling pathways. Transcripts identified and named as B1, B3, B5, B7, B9, B11, B13, B15, B17 and B19 were validated through Nested PCR. Significant difference in the expression pattern of target gene was revealed after normalisation with GAPDH. Gene expression was up-regulated with some exceptions as down-regulated. In silico analysis of HSP70 gene family identified from A. sisalana suggests that genetic transformation of this gene will induce heat tolerance in transgenic crops.
英文关键词	Agave sisalana transcriptome data bioinformatics analyses heat shock responsive gene HSP70 RACE-PCR
类型	Article
语种	英语
国家	Pakistan
收录类别	SCI-E
WOS记录号	WOS:000495277300001
WOS关键词	CRASSULACEAN ACID METABOLISM ; MOLECULAR CHAPERONES ; ARABIDOPSIS ; PLANTS ; ANNOTATION ; TOLERANCE ; BIP ; DNA
WOS类目	Horticulture
WOS研究方向	Agriculture
EI主题词	2019-11-11
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310823
作者单位	Univ Punjab, Ctr Excellence Mol Biol, Lahore, Pakistan
推荐引用方式 GB/T 7714	Batcho, Anicet Agossa,Sarwar, Muhammad Bilal,Tariq, Leeza,et al. Identification and characterisation of heat shock protein gene (HSP70) family and its expression in Agave sisalana under heat stress[J],2020,95(4):470-482.
APA	Batcho, Anicet Agossa,Sarwar, Muhammad Bilal,Tariq, Leeza,Rashid, Bushra,Hassan, Sameera,&Husnain, Tayyab.(2020).Identification and characterisation of heat shock protein gene (HSP70) family and its expression in Agave sisalana under heat stress.JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY,95(4),470-482.
MLA	Batcho, Anicet Agossa,et al."Identification and characterisation of heat shock protein gene (HSP70) family and its expression in Agave sisalana under heat stress".JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY 95.4(2020):470-482.