干旱区生态环境知识资源中心(Arid): Cloning and functional analysis of the FAD2 gene family from desert shrub Artemisia sphaerocephala

Arid

DOI	10.1186/s12870-019-2083-5
	Cloning and functional analysis of the FAD2 gene family from desert shrub Artemisia sphaerocephala
	Miao, Xiumei ; Zhang, Lijing 1; Hu, Xiaowei ; Nan, Shuzhen ; Chen, Xiaolong ; Fu, Hua 1
通讯作者	Zhang, Lijing ; Fu, Hua
来源期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
出版年	2019
卷号	19 期号:1
英文摘要	BackgroundLinoleic acid is an important polyunsaturated fatty acid, required for all eukaryotes. Microsomal delta-12 (Delta(12)) oleate desaturase (FAD2) is a key enzyme for linoleic acid biosynthesis. Desert shrub Artemisia sphaerocephala is rich in linoleic acid, it has a large FAD2 gene family with twenty-six members. The aim of this work is to unveil the difference and potentially functionality of AsFAD2 family members.ResultsFull-length cDNAs of twenty-one AsFAD2 genes were obtained from A. sphaerocephala. The putative polypeptides encoded by AsFAD2 family genes showed a high level of sequence similarity and were relatively conserved during evolution. The motif composition was also relatively conservative. Quantitative real-time PCR analysis revealed that the AsFAD2-1 gene was strongly expressed in developing seeds, which may be closely associated with the high accumulating ability of linoleic acid in A. sphaerocephala seeds. Although different AsFAD2 family members showed diverse response to salt stress, the overall mRNA levels of the AsFAD2 family genes was stable. Transient expression of AsFAD2 genes in the Nicotiana benthamiana leaves revealed that the encoded proteins were all located in the endoplasmic reticulum. Heterologous expression in Saccharomyces cerevisiae suggested that only three AsFAD2 enzymes, AsFAD2-1, -10, and-23, were Delta(12) oleate desaturases, which could convert oleic acid to linoleic acid, whereas AsFAD2-1 and AsFAD2-10 could also produce palmitolinoleic acid.ConclusionsThis research reported the cloning, expression studies, subcellular localization and functional identification of the large AsFAD2 gene family. These results should be helpful in understanding fatty acid biosynthesis in A. sphaerocephala, and has the potential to be applied in the study of plant fatty acids traits.
英文关键词	Fatty acid desaturase linoleic acid expression analysis subcellular localization yeast expression
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	Green Published, Green Submitted, gold
收录类别	SCI-E
WOS记录号	WOS:000495653000002
WOS关键词	FATTY-ACID DESATURASES ; MOLECULAR-CLONING ; LOW-TEMPERATURE ; BRASSICA-NAPUS ; EXPRESSION ; IDENTIFICATION ; PLANTS ; BIOSYNTHESIS ; EVOLUTION ; INSIGHTS
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
EI主题词	2019-11-08
来源机构	兰州大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310785
作者单位	1.Lanzhou Univ, State Key Lab Grassland Agroecosyst, Lanzhou 730020, Gansu, Peoples R China; 2.Lanzhou Univ, Key Lab Grassland Livestock Ind Innovat, Lanzhou 730020, Gansu, Peoples R China; 3.Lanzhou Univ, Minist Agr & Rural Affairs, Lanzhou 730020, Gansu, Peoples R China; 4.Lanzhou Univ, Engn Res Ctr Grassland Ind, Minist Educ, Lanzhou 730020, Gansu, Peoples R China; 5.Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Lanzhou 730020, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Miao, Xiumei,Zhang, Lijing,Hu, Xiaowei,et al. Cloning and functional analysis of the FAD2 gene family from desert shrub Artemisia sphaerocephala[J]. 兰州大学,2019,19(1).
APA	Miao, Xiumei,Zhang, Lijing,Hu, Xiaowei,Nan, Shuzhen,Chen, Xiaolong,&Fu, Hua.(2019).Cloning and functional analysis of the FAD2 gene family from desert shrub Artemisia sphaerocephala.BMC PLANT BIOLOGY,19(1).
MLA	Miao, Xiumei,et al."Cloning and functional analysis of the FAD2 gene family from desert shrub Artemisia sphaerocephala".BMC PLANT BIOLOGY 19.1(2019).