干旱区生态环境知识资源中心(Arid): Insights into non-symbiotic plant growth promotion bacteria associated with nodules of Sphaerophysa salsula growing in northwestern China

Arid

DOI	10.1007/s00203-019-01752-7
	Insights into non-symbiotic plant growth promotion bacteria associated with nodules of Sphaerophysa salsula growing in northwestern China
	Deng, Zhen-Shan 1; Kong, Zhao-Yu 2; Zhang, Bao-Cheng 3; Zhao, Long-Fei 4
通讯作者	Deng, Zhen-Shan
来源期刊	ARCHIVES OF MICROBIOLOGY
ISSN	0302-8933
EISSN	1432-072X
出版年	2020
卷号	202 期号:2 页码:399-409
英文摘要	In addition to rhizobia, other non-symbiotic endophytic bacteria also have been simultaneously isolated from the same root nodules. The existence of non-symbiotic endophytic bacteria in leguminous root nodules is a universal phenomenon. The vast majority of studies have detected endophytic bacteria in other plant tissues. In contrast, little systemic observation has been made on the non-symbiotic endophytic bacteria within leguminous root nodules. The present investigation was carried out to isolate plant growth-promoting endophytic non-symbiotic bacteria from indigenous leguminous Sphaerophysa salsula and their influence on plant growth. A total of 65 endophytic root nodule-associated bacteria were isolated from indigenous legume S. salsula growing in the northwestern arid regions of China. When combining our previous work with the current study, sequence analysis of the nifH gene revealed that the strain belonging to non-nodulating Bacillus pumilus Qtx-10 had genes similar to those of Rhizobium leguminosarum Qtx-10-1. The results indicated that horizontal gene transfer could have occurred between rhizobia and non-symbiotic endophyties. Under pot culture conditions, out of the 20 representative endophytic isolates, 15 with plant growth-promoting traits, such as IAA production, ACC deaminase, phosphate solubilization, chitinase, siderophore, and fungal inhibition activity showed plant growth-promoting activity with respect to various plant parameters such as chlorophyll content, fresh weight of plant, shoot length, nodule number per plant and average nodule weight per plant when co-inoculated with rhizobial bioinoculant Mesorhizobium sp. Zw-19 under N-free culture conditions. Among them, Bacillus pumilus Qtx-10 and Streptomyces bottropensis Gt-10 were excellent plant growth-promoting bacteria, which enhanced the seeding fresh weight by 87.5% and the shoot length by 89.4%, respectively. The number of nodules grew more than 31.89% under field conditions. Our findings indicate the frequent presence of these non-symbiotic endophytic bacteria within root nodules, and that they help to improve nodulation and nitrogen fixation in legume plants through synergistic interactions with rhizobia.
英文关键词	Root nodules Plant growth promotion Phylogeny Non-symbiotic endophytes Sphaerophysa salsula
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000493975100001
WOS关键词	ENDOPHYTIC BACTERIA ; ROOT-NODULES ; BACILLUS STRAINS ; SP NOV. ; GENETIC DIVERSITY ; LEGUMES ; RHIZOBIA ; BURKHOLDERIA ; INOCULATION ; PEA
WOS类目	Microbiology
WOS研究方向	Microbiology
EI主题词	2019-11-04
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310747
作者单位	1.Yanan Univ, Coll Life Sci, Yanan 716000, Peoples R China; 2.Nanchang Univ, Coll Life Sci, Nanchang 330000, Jiangxi, Peoples R China; 3.Zunyi Normal Coll, Sch Biol & Agr Sci Technol, Zunyi 53602, Guizhou, Peoples R China; 4.Shangqiu Normal Univ, Coll Life Sci, Shangqiu 476000, Peoples R China
推荐引用方式 GB/T 7714	Deng, Zhen-Shan,Kong, Zhao-Yu,Zhang, Bao-Cheng,et al. Insights into non-symbiotic plant growth promotion bacteria associated with nodules of Sphaerophysa salsula growing in northwestern China[J],2020,202(2):399-409.
APA	Deng, Zhen-Shan,Kong, Zhao-Yu,Zhang, Bao-Cheng,&Zhao, Long-Fei.(2020).Insights into non-symbiotic plant growth promotion bacteria associated with nodules of Sphaerophysa salsula growing in northwestern China.ARCHIVES OF MICROBIOLOGY,202(2),399-409.
MLA	Deng, Zhen-Shan,et al."Insights into non-symbiotic plant growth promotion bacteria associated with nodules of Sphaerophysa salsula growing in northwestern China".ARCHIVES OF MICROBIOLOGY 202.2(2020):399-409.