干旱区生态环境知识资源中心(Arid): The Salix psammophila SpRLCK1 involved in drought and salt tolerance

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DOI	10.1016/j.plaphy.2019.09.042
	The Salix psammophila SpRLCK1 involved in drought and salt tolerance
	Jia, Huixia 1; Li, Jianbo 2; Zhang, Jin 3; Sun, Pei 1; Lu, Mengzhu 1; Hu, Jianjun 1
通讯作者	Hu, Jianjun
来源期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
出版年	2019
卷号	144 页码:222-233
英文摘要	Receptor-like cytoplasmic kinases (RLCKs) play critical roles in biotic and abiotic stress responses in plants. However, the functions of RLCKs from the desert shrub willow Salix psammophila have not been characterized. Here, we focused on the biological function of SpRLCK1, which was previously identified as a potential drought-related gene. Phylogenetic analysis and subcellular localization revealed that SpRLCK1 was a cytoplasmic-localized protein with a protein kinase domain and belonged to the RLCK VIIa subclass. Gene expression profile revealed that SpRLCK1 was predominantly expressed in the root, being consistent with the GUS staining of pSpRLCK1:GUS transgenic plants. Additionally, the expression of SpRLCK1 was significantly induced by drought and salt stresses. To verify the function of SpRLCK1, we generated its overexpressing transgenic lines in Arabidopsis thaliana. The SpRLCK1-overexpressing plants exhibited higher tolerance to drought and salt stresses, as evidenced by the higher survival rate, relative water content and antioxidant enzyme activity than those of wild-type plants. The SpRLCK1-overexpressing plants enhanced drought and salt tolerance by improving ROS-scavenging activities. A co-expression network for SpRLCK1 was constructed, and the expression analysis indicated that SpRLCK1 regulated the expression of a series of stress-related genes. Taken together, our results demonstrate that SpRLCK1 confers plant drought and salt tolerance through enhancing the activity of antioxidant enzymes and cooperating with stress-related genes.
英文关键词	Receptor-like protein kinase Transgene Drought Salt Antioxidant enzyme Salix psammophila
类型	Article
语种	英语
国家	Peoples R China ; USA
开放获取类型	Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000496039500023
WOS关键词	RECEPTOR-LIKE KINASE ; CYTOPLASMIC KINASE ; TRANSCRIPTION FACTOR ; SIGNAL-TRANSDUCTION ; FUNCTIONAL-ANALYSIS ; ARABIDOPSIS WRKY33 ; STRESS TOLERANCE ; PROTEIN-KINASE ; GENE FAMILY ; CELL-DEATH
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
EI主题词	2019-11-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310611
作者单位	1.Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat Natl Forestry &, Beijing 100091, Peoples R China; 2.Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China; 3.Oak Ridge Natl Lab, Biosci Div, POB 2009, Oak Ridge, TN 37831 USA
推荐引用方式 GB/T 7714	Jia, Huixia,Li, Jianbo,Zhang, Jin,et al. The Salix psammophila SpRLCK1 involved in drought and salt tolerance[J],2019,144:222-233.
APA	Jia, Huixia,Li, Jianbo,Zhang, Jin,Sun, Pei,Lu, Mengzhu,&Hu, Jianjun.(2019).The Salix psammophila SpRLCK1 involved in drought and salt tolerance.PLANT PHYSIOLOGY AND BIOCHEMISTRY,144,222-233.
MLA	Jia, Huixia,et al."The Salix psammophila SpRLCK1 involved in drought and salt tolerance".PLANT PHYSIOLOGY AND BIOCHEMISTRY 144(2019):222-233.