干旱区生态环境知识资源中心(Arid): Effects of FABP knockdown on flight performance of the desert locust, Schistocerca gregaria

Arid

DOI	10.1242/jeb.203455
	Effects of FABP knockdown on flight performance of the desert locust, Schistocerca gregaria
	Rajapakse, Sanjeeva; Qu, David; Ahmed, Ahmed Sayed; Rickers-Haunerland, Jutta; Haunerland, Norbert H.
通讯作者	Haunerland, Norbert H.
来源期刊	JOURNAL OF EXPERIMENTAL BIOLOGY
ISSN	0022-0949
EISSN	1477-9145
出版年	2019
卷号	222 期号:21
英文摘要	During migratory flight, desert locusts rely on fatty acids as their predominant source of energy. Lipids mobilized in the fat body are transported to the flight muscles and enter the muscle cells as free fatty acids. It has been postulated that muscle fatty acid binding protein (FABP) is needed for the efficient translocation of fatty acids through the aqueous cytosol towards mitochondrial beta-oxidation. To assess whether FABP is required for this process, dsRNA was injected into freshly emerged adult males to knock down the expression of FABP. Three weeks after injection, FABP and its mRNA were undetectable in flight muscle, indicating efficient silencing of FABP expression. At rest, control and treated animals exhibited no morphological or behavioral differences. In tethered flight experiments, both control and treated insects were able to fly continually in the initial, carbohydrate-fueled phase of flight, and in both groups, lipids were mobilized and released into the hemolymph. Flight periods exceeding 30 min, however, when fatty acids become the main energy source, were rarely possible for FABP-depleted animals, while control insects continued to fly for more than 2 h. These results demonstrate that FABP is an essential element of skeletal muscle energy metabolism in vivo.
英文关键词	RNAi Fatty acid binding protein Insect flight Lipid transport
类型	Article
语种	英语
国家	Canada
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:000496916700009
WOS关键词	ACID-BINDING-PROTEIN ; SYSTEMIC RNA INTERFERENCE ; DEVELOPMENTAL-CHANGES ; MUSCLE ; EXPRESSION ; GENE ; INSECTS
WOS类目	Biology
WOS研究方向	Life Sciences & Biomedicine - Other Topics
EI主题词	2019-11-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310569
作者单位	Simon Fraser Univ, Dept Biol Sci, Burnaby, BC V5A 1S6, Canada
推荐引用方式 GB/T 7714	Rajapakse, Sanjeeva,Qu, David,Ahmed, Ahmed Sayed,et al. Effects of FABP knockdown on flight performance of the desert locust, Schistocerca gregaria[J],2019,222(21).
APA	Rajapakse, Sanjeeva,Qu, David,Ahmed, Ahmed Sayed,Rickers-Haunerland, Jutta,&Haunerland, Norbert H..(2019).Effects of FABP knockdown on flight performance of the desert locust, Schistocerca gregaria.JOURNAL OF EXPERIMENTAL BIOLOGY,222(21).
MLA	Rajapakse, Sanjeeva,et al."Effects of FABP knockdown on flight performance of the desert locust, Schistocerca gregaria".JOURNAL OF EXPERIMENTAL BIOLOGY 222.21(2019).