干旱区生态环境知识资源中心(Arid): Genome-wide identification and analysis of genes associated with RNA interference in Bemisia tabaci

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DOI	10.1002/ps.5415
	Genome-wide identification and analysis of genes associated with RNA interference in Bemisia tabaci
	Tian, Lixia 1; Zeng, Yang 1; Xie, Wen 1; Wu, Qingjun 1; Wang, Shaoli 1; Zhou, Xuguo 2; Zhang, Youjun 1
通讯作者	Zhang, Youjun
来源期刊	PEST MANAGEMENT SCIENCE
ISSN	1526-498X
EISSN	1526-4998
出版年	2019
卷号	75 期号:11 页码:3005-3014
英文摘要	Background As a method of RNA-mediated gene silencing, RNA interference (RNAi) is a useful reverse genetic tool with which to study gene function, and holds great promise for pest management. Bemisia tabaci is a cosmopolitan pest that causes extensive damage to crops. The mechanism underlying RNAi efficiency in B. tabaci is not well known. We identified and analyzed candidate genes in the RNAi pathway to understand the RNAi mechanism and provide a basis for the application of RNAi in pest management. Results We identified 33 genes putatively involved in the RNAi pathway from the B. tabaci Q genome. Phylogenetic and structural analyses confirmed the characteristics of these genes. Furthermore, quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) and transcriptomic analysis profiled gene expression patterns during different developmental stages. Gene expression levels estimated by qRT-PCR and RNA-seq analyses were significantly correlated. Moreover, gene functions were verified by RNAi. When accompanied by knockdown of AGO2, Dicer2 and Sid1, the efficiency of CYP6DB3 RNAi decreased correspondingly. Conclusion In this study, we annotated and validated genes involved in B. tabaci RNAi. A better understanding of the building blocks of the RNAi process in B. tabaci facilitates integration of this novel biotechnology into the management of this emerging pest, either directly or indirectly. (c) 2019 Society of Chemical Industry
英文关键词	RNAi pathway annotation functional analysis Bemisia tabaci
类型	Article
语种	英语
国家	Peoples R China ; USA
收录类别	SCI-E
WOS记录号	WOS:000488988100019
WOS关键词	DOUBLE-STRANDED-RNA ; PLANT-MEDIATED RNAI ; NUCLEAR EXPORT ; SYSTEMIC RNAI ; ARGONAUTE PROTEINS ; SEX DETERMINATION ; DESERT LOCUST ; PRE-MICRORNAS ; INSECT PESTS ; RESISTANCE
WOS类目	Agronomy ; Entomology
WOS研究方向	Agriculture ; Entomology
EI主题词	2019-11-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310501
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China; 2.Univ Kentucky, Dept Entomol, Lexington, KY USA
推荐引用方式 GB/T 7714	Tian, Lixia,Zeng, Yang,Xie, Wen,et al. Genome-wide identification and analysis of genes associated with RNA interference in Bemisia tabaci[J],2019,75(11):3005-3014.
APA	Tian, Lixia.,Zeng, Yang.,Xie, Wen.,Wu, Qingjun.,Wang, Shaoli.,...&Zhang, Youjun.(2019).Genome-wide identification and analysis of genes associated with RNA interference in Bemisia tabaci.PEST MANAGEMENT SCIENCE,75(11),3005-3014.
MLA	Tian, Lixia,et al."Genome-wide identification and analysis of genes associated with RNA interference in Bemisia tabaci".PEST MANAGEMENT SCIENCE 75.11(2019):3005-3014.