干旱区生态环境知识资源中心(Arid): Transcriptome profiling using RNA-seq to provide insights into foxtail millet seedling tolerance to short-term water deficit stress induced by PEG-6000

Arid

DOI	10.1016/S2095-3119(19)62576-1
	Transcriptome profiling using RNA-seq to provide insights into foxtail millet seedling tolerance to short-term water deficit stress induced by PEG-6000
	Xu Bing-qin; Gao Xiao-li; Gao Jin-feng; Li Jing; Yang Pu; Feng Bai-li
通讯作者	Feng Bai-li
来源期刊	JOURNAL OF INTEGRATIVE AGRICULTURE
ISSN	2095-3119
出版年	2019
卷号	18 期号:11 页码:2457-2471
英文摘要	Foxtail millet (Setaria italica L.) with high drought resistance, is grown widely in arid and semi-arid regions of the world, and it is a new model plant for genetic and molecular studies. To uncover the molecular mechanisms of stress-tolerance in different genotypes of foxtail millet, physiological analyses combined with transcriptional profiling were conducted using a time-course analysis on two foxtail millet genotypes Damaomao (DM) and Hongnian (HN). The genotype DM performed better than HN under water deficiency, with more moderate relative water content (RWC) and chlorophyll decline. Further physiological and RNA-seq investigations revealed that the two genotypes possessed high conservatism in some vital biological pathways which respond to drought stress, involving hormone synthesis, proline, and soluble sugar synthesis, and reactive oxygen species (ROS) metabolism. However, some genes related to these pathways showed different expression profiles. Likewise, the lower malondialdehyde (MDA) content in HN than DM may be explained by the observation that HN contained more activated genes in the ascorbate-glutathione cycle using KEGG pathway analysis. Overall, abscisic acid (ABA) response genes, ROS scavengers which were probably involved in signaling responses, a set of enzymes involved in proline and soluble sugar synthesis, channel protein genes, and transcription factors, encompassed the early strategy of foxtail millet response to drought. These findings provide a comprehensive molecular view of how different foxtail millet genotypes respond to short-term osmotic stress.
英文关键词	transcriptome foxtail millet osmotic stress RNA-seq gene expression
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:000495043700003
WOS关键词	PLANT-RESPONSES ; SALINITY STRESS ; DROUGHT STRESS ; PROLINE ; GENES
WOS类目	Agriculture, Multidisciplinary
WOS研究方向	Agriculture
EI主题词	2019-11-01
来源机构	西北农林科技大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310495
作者单位	Northwest A&F Univ, Coll Agron, State Key Lab Crop Stress Biol Arid Areas, Minist Sci & Technol, Yangling 712100, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Xu Bing-qin,Gao Xiao-li,Gao Jin-feng,et al. Transcriptome profiling using RNA-seq to provide insights into foxtail millet seedling tolerance to short-term water deficit stress induced by PEG-6000[J]. 西北农林科技大学,2019,18(11):2457-2471.
APA	Xu Bing-qin,Gao Xiao-li,Gao Jin-feng,Li Jing,Yang Pu,&Feng Bai-li.(2019).Transcriptome profiling using RNA-seq to provide insights into foxtail millet seedling tolerance to short-term water deficit stress induced by PEG-6000.JOURNAL OF INTEGRATIVE AGRICULTURE,18(11),2457-2471.
MLA	Xu Bing-qin,et al."Transcriptome profiling using RNA-seq to provide insights into foxtail millet seedling tolerance to short-term water deficit stress induced by PEG-6000".JOURNAL OF INTEGRATIVE AGRICULTURE 18.11(2019):2457-2471.