干旱区生态环境知识资源中心(Arid): PeSTZ1, a C2H2-type zinc finger transcription factor from Populus euphratica, enhances freezing tolerance through modulation of ROS scavenging by directly regulating PeAPX2

Arid

DOI	10.1111/pbi.13130
	PeSTZ1, a C2H2-type zinc finger transcription factor from Populus euphratica, enhances freezing tolerance through modulation of ROS scavenging by directly regulating PeAPX2
	He, Fang; Li, Hui-Guang; Wang, Jing-Jing; Su, Yanyan; Wang, Hou-Ling; Feng, Cong-Hua; Yang, Yanli; Niu, Meng-Xue; Liu, Chao; Yin, Weilun; Xia, Xinli
通讯作者	Xia, Xinli
来源期刊	PLANT BIOTECHNOLOGY JOURNAL
ISSN	1467-7644
EISSN	1467-7652
出版年	2019
卷号	17 期号:11 页码:2169-2183
英文摘要	In the present study, PeSTZ1, a cysteine-2/histidine-2-type zinc finger transcription factor, was isolated from the desert poplar, Populus euphratica, which serves as a model stress adaptation system for trees. PeSTZ1 was preferentially expressed in the young stems and was significantly up-regulated during chilling and freezing treatments. PeSTZ1 was localized to the nucleus and bound specifically to the PeAPX2 promoter. To examine the potential functions of PeSTZ1, we overexpressed it in poplar 84K hybrids (Populus alba x Populus glandulosa), which are known to be stress-sensitive. Upon exposure to freezing stress, transgenic poplars maintained higher photosynthetic activity and dissipated more excess light energy (in the form of heat) than wild-type poplars. Thus, PeSTZ1 functions as a transcription activator to enhance freezing tolerance without sacrificing growth. Under freezing stress, PeSTZ1 acts upstream of ASCORBATE PEROXIDASE2 (PeAPX2) and directly regulates its expression by binding to its promoter. Activated PeAPX2 promotes cytosolic APX that scavenges reactive oxygen species (ROS) under cold stress. PeSTZ1 may operate in parallel with C-REPEAT-BINDING FACTORS to regulate COLD-REGULATED gene expression. Moreover, PeSTZ1 up-regulation reduces malondialdehyde and ROS accumulation by activating the antioxidant system. Taken together, these results suggested that overexpressing PeSTZ1 in 84K poplar enhances freezing tolerance through the modulation of ROS scavenging via the direct regulation of PeAPX2 expression.
英文关键词	freezing stress poplar PeSTZ1 PeAPX2 reactive oxygen species transgenic
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000492276200015
WOS关键词	ABIOTIC STRESS TOLERANCE ; LOW-TEMPERATURE ; DROUGHT TOLERANCE ; GENE-EXPRESSION ; COLD TOLERANCE ; SALT TOLERANCE ; ARABIDOPSIS ; PROTEIN ; OVEREXPRESSION ; PHOTOSYNTHESIS
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
EI主题词	2019-11-01
来源机构	北京林业大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310492
作者单位	Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Lab Tree Breeding, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing, Peoples R China
推荐引用方式 GB/T 7714	He, Fang,Li, Hui-Guang,Wang, Jing-Jing,et al. PeSTZ1, a C2H2-type zinc finger transcription factor from Populus euphratica, enhances freezing tolerance through modulation of ROS scavenging by directly regulating PeAPX2[J]. 北京林业大学,2019,17(11):2169-2183.
APA	He, Fang.,Li, Hui-Guang.,Wang, Jing-Jing.,Su, Yanyan.,Wang, Hou-Ling.,...&Xia, Xinli.(2019).PeSTZ1, a C2H2-type zinc finger transcription factor from Populus euphratica, enhances freezing tolerance through modulation of ROS scavenging by directly regulating PeAPX2.PLANT BIOTECHNOLOGY JOURNAL,17(11),2169-2183.
MLA	He, Fang,et al."PeSTZ1, a C2H2-type zinc finger transcription factor from Populus euphratica, enhances freezing tolerance through modulation of ROS scavenging by directly regulating PeAPX2".PLANT BIOTECHNOLOGY JOURNAL 17.11(2019):2169-2183.