干旱区生态环境知识资源中心(Arid): Effects of recombinant bovine interleukin-8 (rblL-8) treatment on health, metabolism, and lactation performance in Holstein cattle III: Administration of rblL-8 induces insulin resistance in bull calves

Arid

DOI	10.3168/jds.2019-16336
	Effects of recombinant bovine interleukin-8 (rblL-8) treatment on health, metabolism, and lactation performance in Holstein cattle III: Administration of rblL-8 induces insulin resistance in bull calves
	Zinicola, M.1; Menta, P. R.1; Ribeiro, B. L.1; Boisclair, Y.2; Bicalho, R. C.1
通讯作者	Bicalho, R. C.
来源期刊	JOURNAL OF DAIRY SCIENCE
ISSN	0022-0302
EISSN	1525-3198
出版年	2019
卷号	102 期号:11 页码:10329-10339
英文摘要	Our previous work has suggested that recombinant bovine interleukin-8 (rbIL-8) treatment might influence cow metabolism. Therefore, this study was conducted to initially assess the effects of systemic administration of rbIL-8 on response to a glucose challenge, blood metabolites, insulin, growth hormone, insulin-like growth factor-1, immune cell populations, and inflammatory parameters in Holstein bull calves. Calves from 30 +/- 6 d of life were individually housed and randomly allocated to 1 of 2 treatment groups: rbIL-8 (rbIL-8, n = 10) and control (CTR, n = 8). Calves assigned to the rbIL-8 group received 1 s.c. injection (d 1, 0900 h) and 6 i.v. injections (d 1 at 1600 h, d 2 and 3 at 0900 h and 1600 h, and d 4 at 0900 h) of rbIL-8 (4 mu g/kg of body weight), whereas the CTR group received 2 mL of sterile saline solution at each time point. Day of enrollment was considered as d 1, and the study duration was 10 d. Insulin concentrations and whole-body glucose disappearance were evaluated by an i.v. glucose tolerance test conducted at 12 h and 7 d following the last rbIL-8 injection. Rectal temperature and blood samples were collected on d 1, 2, 3, and 4 at -30 (before treatmerit, 0830 h), 30, 60, 120, 240, arid 360 min relative to treatment, and daily at 0830 h for the rest of the study period. Serum was harvested, and the following parameters were measured: beta-hydroxybutyrate (BHB), nonesterified fatty acids, glucose, insulin, plasma urea nitrogen, haptoglobin, and differential blood count. Significant differences were considered when P <= 0.05 and a trend if 0.05 < P <= 0.10. Serum glucose levels and glucose area under the curve (AUC) did riot differ between treatment groups in response to the glucose challenge. However, calves treated with rbIL-8 had greater serum insulin concentration and insulin AUC compared with controls. Administration of rbIL-8 increased rectal temperature (rbIL-8 = 39.3 +/- 0.1; CTR = 38.9 +/- 0.1 degrees C; +/- standard error), BHB concentrations (rbIL-8 = 3.54 +/- 0.10; CTR = 2.99 +/- 0.12 mg/dL), counts of lymphocytes (rbIL-8 = 4.52 +/- 0.12; CTR = 3.84 +/- 0.14 x 10(3)/mu L), monocytes (rbIL-8 = 0.87 +/- 0.03; CTR = 0.67 +/- 0.04 x 10(3)/mu L), and granulocytes (rbIL-8 = 3.54 + 0.22; CTR. = 2.66 + 0.24 x 10(3)/mu L). We conclude that rbIL-8 induces insulin resistance in Holstein bull calves, accompanied by systemic inflammation and altered blood metabolites and white blood cell populations.
英文关键词	chemokine insulin sensitivity inflanimation
类型	Article
语种	英语
国家	USA
开放获取类型	Bronze
收录类别	SCI-E
WOS记录号	WOS:000489285000062
WOS关键词	NEGATIVE-ENERGY BALANCE ; ADIPOSE-TISSUE ; DAIRY-COWS ; GH RESPONSIVENESS ; GROWTH-HORMONE ; MUSCLE ATROPHY ; TECHNICAL NOTE ; DRY PERIOD ; GLUCOSE ; TRANSITION
WOS类目	Agriculture, Dairy & Animal Science ; Food Science & Technology
WOS研究方向	Agriculture ; Food Science & Technology
EI主题词	2019-11-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310473
作者单位	1.Cornell Univ, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA; 2.Cornell Univ, Dept Anim Sci, Ithaca, NY 14853 USA
推荐引用方式 GB/T 7714	Zinicola, M.,Menta, P. R.,Ribeiro, B. L.,et al. Effects of recombinant bovine interleukin-8 (rblL-8) treatment on health, metabolism, and lactation performance in Holstein cattle III: Administration of rblL-8 induces insulin resistance in bull calves[J],2019,102(11):10329-10339.
APA	Zinicola, M.,Menta, P. R.,Ribeiro, B. L.,Boisclair, Y.,&Bicalho, R. C..(2019).Effects of recombinant bovine interleukin-8 (rblL-8) treatment on health, metabolism, and lactation performance in Holstein cattle III: Administration of rblL-8 induces insulin resistance in bull calves.JOURNAL OF DAIRY SCIENCE,102(11),10329-10339.
MLA	Zinicola, M.,et al."Effects of recombinant bovine interleukin-8 (rblL-8) treatment on health, metabolism, and lactation performance in Holstein cattle III: Administration of rblL-8 induces insulin resistance in bull calves".JOURNAL OF DAIRY SCIENCE 102.11(2019):10329-10339.