干旱区生态环境知识资源中心(Arid): Noninvasive Identification of Herpetofauna: Pairing Conservation Dogs and Genetic Analysis

Arid

DOI	10.1002/jwmg.21772
	Noninvasive Identification of Herpetofauna: Pairing Conservation Dogs and Genetic Analysis
	Statham, Mark J.1; Woollett, Deborah A. (Smith)2; Fresquez, Susan 3; Pfeiffer, Jerene 3; Richmond, Jonathan 4; Whitelaw, Alice 2; Richards, Ngaio L.2; Westphal, Michael F.5; Sacks, Benjamin N.1
通讯作者	Statham, Mark J.
来源期刊	JOURNAL OF WILDLIFE MANAGEMENT
ISSN	0022-541X
EISSN	1937-2817
出版年	2020
卷号	84 期号:1 页码:66-74
英文摘要	Noninvasive fecal sampling combined with genetic analysis is a technique allowing the study of elusive or otherwise difficult to monitor species without the need for direct contact. Although this method is widely used in birds and mammals, it has never been successfully applied on a large scale in reptiles. The blunt-nosed leopard lizard (Gambelia sila) is an endangered species endemic to the San Joaquin Desert, California, USA. Presently, acquiring data on the species for research and management involves more traditional methods such as live capture or visual surveys, the latter of which are required for regulatory monitoring in accordance with wildlife agency protocols. We used an approach for gathering additional information that combines conservation detection dogs trained to locate blunt-nosed leopard lizard scat samples with genetic analysis for identifying and distinguishing among sympatric lizard species. We developed 2 polymerase chain reaction assays that produce fluorescently labelled amplicons of species-specific fragment length for 6 lizard species in the study area. Using these assays, we genetically identified to species 78% (255 of 327) of samples collected by dog-handler teams across 4 years. The majority of the genetically identified samples (82.4%; 210 of 255) were confirmed as originating from blunt-nosed leopard lizards. Although an assessment of the viability of detection dogs in regulatory monitoring efforts is required, our ability to recover usable DNA and to differentiate among a diverse group of lizards highlights the broad potential of our methodology for noninvasive sampling in reptiles. (c) 2019 The Authors. The Journal of Wildlife Management published by Wiley Periodicals, Inc. on behalf of The Wildlife Society.
英文关键词	blunt-nosed leopard lizard conservation detection dog Gambelia sila genetic analysis population monitoring reptile San Joaquin Desert scat species identification western whiptail lizard
类型	Article
语种	英语
国家	USA
开放获取类型	hybrid
收录类别	SCI-E
WOS记录号	WOS:000493182800001
WOS关键词	SCAT-DETECTION DOGS ; JOAQUIN KIT FOXES ; DNA ; FECES ; POPULATION ; CALIFORNIA ; DENSITY ; LIZARDS ; BLACK
WOS类目	Ecology ; Zoology
WOS研究方向	Environmental Sciences & Ecology ; Zoology
EI主题词	2019-10-30
来源机构	United States Geological Survey ; University of California, Davis
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310386
作者单位	1.Univ Calif Davis, Vet Genet Lab, Mammalian Ecol & Conservat Unit, Dept Populat Hlth & Reprod,Sch Vet Med, One Shields Ave, Davis, CA 95616 USA; 2.Working Dogs Conservat, POB 280, Bozeman, MT 59771 USA; 3.Univ Calif Davis, Vet Genet Lab, Mammalian Ecol & Conservat Unit, One Shields Ave, Davis, CA 95616 USA; 4.US Geol Survey, 4165 Spruance Rd,Suite 200, San Diego, CA 92101 USA; 5.US Bur Land Management, Cent Coast Field Off, Marina, CA 93933 USA
推荐引用方式 GB/T 7714	Statham, Mark J.,Woollett, Deborah A. ,Fresquez, Susan,et al. Noninvasive Identification of Herpetofauna: Pairing Conservation Dogs and Genetic Analysis[J]. United States Geological Survey, University of California, Davis,2020,84(1):66-74.
APA	Statham, Mark J..,Woollett, Deborah A. .,Fresquez, Susan.,Pfeiffer, Jerene.,Richmond, Jonathan.,...&Sacks, Benjamin N..(2020).Noninvasive Identification of Herpetofauna: Pairing Conservation Dogs and Genetic Analysis.JOURNAL OF WILDLIFE MANAGEMENT,84(1),66-74.
MLA	Statham, Mark J.,et al."Noninvasive Identification of Herpetofauna: Pairing Conservation Dogs and Genetic Analysis".JOURNAL OF WILDLIFE MANAGEMENT 84.1(2020):66-74.