干旱区生态环境知识资源中心(Arid): Knockout of the HaREase Gene Improves the Stability of dsRNA and Increases the Sensitivity of Helicoverpa armigera to Bacillus thuringiensis Toxin

Arid

DOI	10.3389/fphys.2019.01368
	Knockout of the HaREase Gene Improves the Stability of dsRNA and Increases the Sensitivity of Helicoverpa armigera to Bacillus thuringiensis Toxin
	Guan, Ruobing 1,2; Chen, Qiuyan 3; Li, Haichao 1,2; Hu, Shaoru 2,4; Miao, Xuexia 2; Wang, Guirong 3; Yang, Bin 3
通讯作者	Guan, Ruobing ; Yang, Bin
来源期刊	FRONTIERS IN PHYSIOLOGY
ISSN	1664-042X
出版年	2019
卷号	10
英文摘要	Double-stranded RNA (dsRNA)-induced genes are usually related to RNA interference (RNAi) mechanisms and are involved in immune-related pathways. In a previous study, we found a lepidopteran-specific nuclease gene REase that was up-regulated by dsRNA and that affected RNAi efficiency in Asian corn borer (Ostrinia furnacalis). In this study, to verify the function of REase, the homologous gene HaREase in cotton bollworm (Helicoverpa armigera) was knocked out using CRISPR/Cas9 system. We found that the midgut epithelium structure was apparently not affected in the Delta HaREase mutant [Knock out (KO)]. Transcript sequencing results showed that most of the known insect immune-related genes were up-regulated in KO. When second instar larvae were fed artificial diet with Cry1Ac, a protoxin from Bacillus thuringiensis (Bt), in sublethal doses (2.5 or 4 mu g/g), the growth rate of KO was repressed significantly. The dsRNA stability was also enhanced in midgut extraction of KO; however, RNAi efficiency was not obviously improved compared with the wild type (WT). The KO and WT were injected with dsEGFP (Enhanced green fluorescent protein) and subjected to transcriptome sequencing. The results showed that the expression levels of 14 nuclease genes were enhanced in KO after the dsRNA treatment. These findings revealed that HaREase expression level was not only related with dsRNA stability, but also with Bt resistance in cotton bollworm. When HaREase was knocked out, other immune- or nuclease-related genes were enhanced significantly. These results remind us that insect immune system is complex and pest control for cotton bollworm is an arduous task.
英文关键词	HaREase CRISPR Cas9 system RNAi efficiency Bt resistance dsRNA stability insect immune
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000494440500001
WOS关键词	RNA INTERFERENCE ; DESERT LOCUST ; RESISTANCE ; LEPIDOPTERA ; TRANSPORTER ; GUT ; DEGRADATION ; EXPRESSION ; EFFICIENCY ; SILKWORM
WOS类目	Physiology
WOS研究方向	Physiology
EI主题词	2019-10-25
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310356
作者单位	1.Henan Agr Univ, State Key Lab Wheat & Maize Crop Sci, Coll Plant Protect, Zhengzhou, Henan, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Key Lab Insect Dev & Evolutionary Biol, Shanghai, Peoples R China; 3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing, Peoples R China; 4.Shanghai Jiao Tong Univ, Sch Med, Biobank Peoples Hosp 9, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Guan, Ruobing,Chen, Qiuyan,Li, Haichao,et al. Knockout of the HaREase Gene Improves the Stability of dsRNA and Increases the Sensitivity of Helicoverpa armigera to Bacillus thuringiensis Toxin[J],2019,10.
APA	Guan, Ruobing.,Chen, Qiuyan.,Li, Haichao.,Hu, Shaoru.,Miao, Xuexia.,...&Yang, Bin.(2019).Knockout of the HaREase Gene Improves the Stability of dsRNA and Increases the Sensitivity of Helicoverpa armigera to Bacillus thuringiensis Toxin.FRONTIERS IN PHYSIOLOGY,10.
MLA	Guan, Ruobing,et al."Knockout of the HaREase Gene Improves the Stability of dsRNA and Increases the Sensitivity of Helicoverpa armigera to Bacillus thuringiensis Toxin".FRONTIERS IN PHYSIOLOGY 10(2019).