Knowledge Resource Center for Ecological Environment in Arid Area
| DOI | 10.3389/fpls.2019.01319 |
| Management of Pest Insects and Plant Diseases by Non-Transformative RNAi | |
| Cagliari, Deise1; Dias, Nayma P.1; Galdeano, Diogo Manzano2; dos Santos, Ericmar Avila1; Smagghe, Guy3; Zotti, Moises Joao1 | |
| 通讯作者 | Cagliari, Deise ; Smagghe, Guy ; Zotti, Moises Joao |
| 来源期刊 | FRONTIERS IN PLANT SCIENCE
 |
| ISSN | 1664-462X |
| 出版年 | 2019 |
| 卷号 | 10 |
| 英文摘要 | Since the discovery of RNA interference (RNAi), scientists have made significant progress towards the development of this unique technology for crop protection. The RNAi mechanism works at the mRNA level by exploiting a sequence-dependent mode of action with high target specificity due to the design of complementary dsRNA molecules, allowing growers to target pests more precisely compared to conventional agrochemicals. The delivery of RNAi through transgenic plants is now a reality with some products currently in the market. Conversely, it is also expected that more RNA-based products reach the market as non-transformative alternatives. For instance, topically applied dsRNA/siRNA (SIGS - Spray Induced Gene Silencing) has attracted attention due to its feasibility and low cost compared to transgenic plants. Once on the leaf surface, dsRNAs can move directly to target pest cells (e.g., insects or pathogens) or can be taken up indirectly by plant cells to then be transferred into the pest cells. Water-soluble formulations containing pesticidal dsRNA provide alternatives, especially in some cases where plant transformation is not possible or takes years and cost millions to be developed (e.g., perennial crops). The ever-growing understanding of the RNAi mechanism and its limitations has allowed scientists to develop non-transgenic approaches such as trunk injection, soaking, and irrigation. While the technology has been considered promising for pest management, some issues such as RNAi efficiency, dsRNA degradation, environmental risk assessments, and resistance evolution still need to be addressed. Here, our main goal is to review some possible strategies for non-transgenic delivery systems, addressing important issues related to the use of this technology. |
| 英文关键词 | RNAi non-transgenic RNAi RNA-based products gene silencing pest insects plant diseases |
| 类型 | Review |
| 语种 | 英语 |
| 国家 | Brazil ; Belgium |
| 开放获取类型 | gold, Green Published |
| 收录类别 | SCI-E |
| WOS记录号 | WOS:000495076800001 |
| WOS关键词 | DOUBLE-STRANDED-RNA ; WESTERN CORN-ROOTWORM ; EXOGENOUS APPLICATION ; ENVIRONMENTAL RNAI ; DSRNA DEGRADATION ; GENE-REGULATION ; DESERT LOCUST ; CROP LOSSES ; INTERFERENCE ; RESISTANCE |
| WOS类目 | Plant Sciences |
| WOS研究方向 | Plant Sciences |
| EI主题词 | 2019-10-25 |
| 资源类型 | 期刊论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/310355 |
| 作者单位 | 1.Fed Univ Pelota, Lab Mol Entomol, Dept Crop Protect, Pelotas, Brazil; 2.Campinas Agron Inst IAC, Sylvio Moreira Citrus Ctr, Cordeiropolis, Brazil; 3.Univ Ghent, Dept Plants & Crops, Ghent, Belgium |
| 推荐引用方式 GB/T 7714 | Cagliari, Deise,Dias, Nayma P.,Galdeano, Diogo Manzano,et al. Management of Pest Insects and Plant Diseases by Non-Transformative RNAi[J],2019,10. |
| APA | Cagliari, Deise,Dias, Nayma P.,Galdeano, Diogo Manzano,dos Santos, Ericmar Avila,Smagghe, Guy,&Zotti, Moises Joao.(2019).Management of Pest Insects and Plant Diseases by Non-Transformative RNAi.FRONTIERS IN PLANT SCIENCE,10. |
| MLA | Cagliari, Deise,et al."Management of Pest Insects and Plant Diseases by Non-Transformative RNAi".FRONTIERS IN PLANT SCIENCE 10(2019). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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