干旱区生态环境知识资源中心(Arid): Development of SSR markers for Astragalus lehmannianus, a vulnerable species from northwestern China

Arid

DOI	10.1002/aps3.11297
	Development of SSR markers for Astragalus lehmannianus, a vulnerable species from northwestern China
	Wu, Mingying 1,2; Shi, Xiaojun 1,2; Tan, Dunyan 1,2,3
通讯作者	Tan, Dunyan
来源期刊	APPLICATIONS IN PLANT SCIENCES
ISSN	2168-0450
出版年	2019
卷号	7 期号:10
英文摘要	Premise Astragalus lehmannianus (Fabaceae) is a vulnerable species found in the cold deserts of northwestern China. We aimed to characterize polymorphic microsatellite loci for A. lehmannianus to support future studies of population genetic dynamics and conservation management of the species. Methods and Results We used next-generation sequencing to detect polymorphic microsatellites. Twenty-five potential microsatellite loci were identified, 12 of which were polymorphic and present in the three study populations of A. lehmannianus. Levels of observed and expected heterozygosities were 0.000-1.000 and 0.000-0.827, respectively. Furthermore, two and five of the 12 developed primers were successfully amplified in two congeneric species, A. arpilobus and A. oxyglottis, respectively. Conclusions These newly developed microsatellite markers can be used to determine population diversity and to develop conservation strategies in A. lehmannianus.
英文关键词	Astragalus lehmannianus conservation Fabacaeae microsatellite marker vulnerable species
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000492088800001
WOS关键词	GENETIC DIVERSITY ; FABACEAE ; SOFTWARE
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
EI主题词	2019-10-24
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310344
作者单位	1.Xinjiang Agr Univ, Xinjiang Key Lab Grassland Resources & Ecol, Coll Grassland & Environm Sci, Urumqi 830052, Peoples R China; 2.Xinjiang Agr Univ, Key Lab Western Arid Reg Grassland Resources & Ec, Minist Educ, Urumqi 830052, Peoples R China; 3.Jishou Univ, Coll Biol & Environm Sci, Jishou 416000, Peoples R China
推荐引用方式 GB/T 7714	Wu, Mingying,Shi, Xiaojun,Tan, Dunyan. Development of SSR markers for Astragalus lehmannianus, a vulnerable species from northwestern China[J],2019,7(10).
APA	Wu, Mingying,Shi, Xiaojun,&Tan, Dunyan.(2019).Development of SSR markers for Astragalus lehmannianus, a vulnerable species from northwestern China.APPLICATIONS IN PLANT SCIENCES,7(10).
MLA	Wu, Mingying,et al."Development of SSR markers for Astragalus lehmannianus, a vulnerable species from northwestern China".APPLICATIONS IN PLANT SCIENCES 7.10(2019).