Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.7717/peerj.7894 |
Manure application increased denitrifying gene abundance in a drip-irrigated cotton field | |
Yin, Mingyuan1,2,3; Gao, Xiaopeng1,2,4; Tenuta, Mario4; Kuang, Wennong1,2,3; Gui, Dongwei1,2; Zeng, Fanjiang1,2![]() | |
通讯作者 | Gao, Xiaopeng |
来源期刊 | PEERJ
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ISSN | 2167-8359 |
出版年 | 2019 |
卷号 | 7 |
英文摘要 | Application of inorganic nitrogen (N) fertilizer and manure can increase nitrous oxide (N2O) emissions. We tested the hypothesis that increased N2O flux from soils amended with manure reflects a change in bacterial community structure and, specifically, an increase in the number of denitrifiers. To test this hypothesis, a field experiment was conducted in a drip-irrigated cotton field in an arid region of northwestern China. Treatments included plots that were not amended (Control), and plots amended with urea (Urea), animal manure (Manure) and a 50/50 mix of urea and manure (U+M). Manure was broadcast-incorporated into the soil before seeding while urea was split-applied with drip irrigation (fertigation) over the growing season. The addition treatments did not, as assessed by nextgen sequencing of PCR-amplicons generated from rRNA genes in soil, affect the alpha diversity of bacterial communities but did change the beta diversity. Compared to the Control, the addition of manure (U+M and Manure) significantly increased the abundance of genes associated with nitrate reduction (narG) and denitrfication (nirK and nosZ). Manure addition (U+M and Manure) did not affect the nitrifying enzyme activity (NEA) of soil but resulted in 39-59 times greater denitrifying enzyme activity (DEA). In contrast, urea application had no impact on the abundances of nitrifier and denitrifier genes, DEA and NEA; likely due to a limitation of C availability. DEA was highly correlated (r = 0.70-0.84, P < 0.01) with the abundance of genes narG, nirK and nosZ. An increase in the abundance of these functional genes was further correlated with soil NO3-, dissolved organic carbon, total C, and total N concentrations, and soil C:N ratio. These results demonstrated a positive relationship between the abundances of denitrifying functional genes (narG, nirK and nosZ) and denitrification potential, suggesting that manure application increased N2O emission by increasing denitrification and the population of bacteria that mediated that process. |
英文关键词 | Bacterial community structure Denitrifier Denitrifying enzyme activity Drip irrigation Functional genes Manure Nitrifier |
类型 | Article |
语种 | 英语 |
国家 | Peoples R China ; Canada |
开放获取类型 | Green Published, gold |
收录类别 | SCI-E |
WOS记录号 | WOS:000491858000005 |
WOS关键词 | AMMONIA-OXIDIZING BACTERIA ; NITROUS-OXIDE EMISSION ; FERTILIZER APPLICATION ; MICROBIAL COMMUNITIES ; ORGANIC FERTILIZERS ; CALCAREOUS SOIL ; PCR PRIMERS ; NOSZ GENES ; DIVERSITY ; DENITRIFICATION |
WOS类目 | Multidisciplinary Sciences |
WOS研究方向 | Science & Technology - Other Topics |
EI主题词 | 2019-10-23 |
来源机构 | 中国科学院新疆生态与地理研究所 |
资源类型 | 期刊论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/310339 |
作者单位 | 1.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi, Peoples R China; 2.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Cele Natl Stn Observat & Res Desert Grassland Eco, Cele, Peoples R China; 3.Univ Chinese Acad Sci, Beijing, Peoples R China; 4.Univ Manitoba, Dept Soil Sci, Winnipeg, MB, Canada |
推荐引用方式 GB/T 7714 | Yin, Mingyuan,Gao, Xiaopeng,Tenuta, Mario,et al. Manure application increased denitrifying gene abundance in a drip-irrigated cotton field[J]. 中国科学院新疆生态与地理研究所,2019,7. |
APA | Yin, Mingyuan,Gao, Xiaopeng,Tenuta, Mario,Kuang, Wennong,Gui, Dongwei,&Zeng, Fanjiang.(2019).Manure application increased denitrifying gene abundance in a drip-irrigated cotton field.PEERJ,7. |
MLA | Yin, Mingyuan,et al."Manure application increased denitrifying gene abundance in a drip-irrigated cotton field".PEERJ 7(2019). |
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