Arid
DOI10.7717/peerj.7894
Manure application increased denitrifying gene abundance in a drip-irrigated cotton field
Yin, Mingyuan1,2,3; Gao, Xiaopeng1,2,4; Tenuta, Mario4; Kuang, Wennong1,2,3; Gui, Dongwei1,2; Zeng, Fanjiang1,2
通讯作者Gao, Xiaopeng
来源期刊PEERJ
ISSN2167-8359
出版年2019
卷号7
英文摘要Application of inorganic nitrogen (N) fertilizer and manure can increase nitrous oxide (N2O) emissions. We tested the hypothesis that increased N2O flux from soils amended with manure reflects a change in bacterial community structure and, specifically, an increase in the number of denitrifiers. To test this hypothesis, a field experiment was conducted in a drip-irrigated cotton field in an arid region of northwestern China. Treatments included plots that were not amended (Control), and plots amended with urea (Urea), animal manure (Manure) and a 50/50 mix of urea and manure (U+M). Manure was broadcast-incorporated into the soil before seeding while urea was split-applied with drip irrigation (fertigation) over the growing season. The addition treatments did not, as assessed by nextgen sequencing of PCR-amplicons generated from rRNA genes in soil, affect the alpha diversity of bacterial communities but did change the beta diversity. Compared to the Control, the addition of manure (U+M and Manure) significantly increased the abundance of genes associated with nitrate reduction (narG) and denitrfication (nirK and nosZ). Manure addition (U+M and Manure) did not affect the nitrifying enzyme activity (NEA) of soil but resulted in 39-59 times greater denitrifying enzyme activity (DEA). In contrast, urea application had no impact on the abundances of nitrifier and denitrifier genes, DEA and NEA; likely due to a limitation of C availability. DEA was highly correlated (r = 0.70-0.84, P < 0.01) with the abundance of genes narG, nirK and nosZ. An increase in the abundance of these functional genes was further correlated with soil NO3-, dissolved organic carbon, total C, and total N concentrations, and soil C:N ratio. These results demonstrated a positive relationship between the abundances of denitrifying functional genes (narG, nirK and nosZ) and denitrification potential, suggesting that manure application increased N2O emission by increasing denitrification and the population of bacteria that mediated that process.
英文关键词Bacterial community structure Denitrifier Denitrifying enzyme activity Drip irrigation Functional genes Manure Nitrifier
类型Article
语种英语
国家Peoples R China ; Canada
开放获取类型Green Published, gold
收录类别SCI-E
WOS记录号WOS:000491858000005
WOS关键词AMMONIA-OXIDIZING BACTERIA ; NITROUS-OXIDE EMISSION ; FERTILIZER APPLICATION ; MICROBIAL COMMUNITIES ; ORGANIC FERTILIZERS ; CALCAREOUS SOIL ; PCR PRIMERS ; NOSZ GENES ; DIVERSITY ; DENITRIFICATION
WOS类目Multidisciplinary Sciences
WOS研究方向Science & Technology - Other Topics
EI主题词2019-10-23
来源机构中国科学院新疆生态与地理研究所
资源类型期刊论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/310339
作者单位1.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi, Peoples R China;
2.Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Cele Natl Stn Observat & Res Desert Grassland Eco, Cele, Peoples R China;
3.Univ Chinese Acad Sci, Beijing, Peoples R China;
4.Univ Manitoba, Dept Soil Sci, Winnipeg, MB, Canada
推荐引用方式
GB/T 7714
Yin, Mingyuan,Gao, Xiaopeng,Tenuta, Mario,et al. Manure application increased denitrifying gene abundance in a drip-irrigated cotton field[J]. 中国科学院新疆生态与地理研究所,2019,7.
APA Yin, Mingyuan,Gao, Xiaopeng,Tenuta, Mario,Kuang, Wennong,Gui, Dongwei,&Zeng, Fanjiang.(2019).Manure application increased denitrifying gene abundance in a drip-irrigated cotton field.PEERJ,7.
MLA Yin, Mingyuan,et al."Manure application increased denitrifying gene abundance in a drip-irrigated cotton field".PEERJ 7(2019).
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