干旱区生态环境知识资源中心(Arid): Comprehensive analysis of bZIP transcription factors uncovers their roles during dimorphic floret differentiation and stress response in Cleistogenes songorica

Arid

DOI	10.1186/s12864-019-6092-4
	Comprehensive analysis of bZIP transcription factors uncovers their roles during dimorphic floret differentiation and stress response in Cleistogenes songorica
	Yan, Qi; Wu, Fan; Ma, Tiantian; Zong, Xifang; Ma, Qian; Li, Jie; Zhao, Yufeng; Wang, Yanrong; Zhang, Jiyu
通讯作者	Zhang, Jiyu
来源期刊	BMC GENOMICS
ISSN	1471-2164
出版年	2019
卷号	20 期号:1
英文摘要	Background Transcription factors act as important regulators of transcription networks. Basic leucine zipper (bZIP) transcription factors have been shown to be involved in multiple biological processes in plants. However, no information is available for the bZIP family in Cleistogenes songorica, which is an important xerophytic and allotetraploid grass in desert grasslands. Results In this study, 86 CsbZIPs were identified in the allotetraploid C. songorica genome. For location analysis, CsbZIPs were distributed evenly across two subgenomes of C. songorica. Phylogenetic tree analysis among three species indicated that CsbZIPs were evolutionarily more closely related to OsbZIPs than AtbZIPs. Syntenic and phylogenetic analyses confirmed that the CsbZIPs were mainly expanded by whole-genome duplication events. Furthermore, it was determined that rice and C. songorica might have undergone purified selection during their long evolutionary history by calculating the Ks values and Ka/Ks ratios of orthologous gene pairs. By analysing the expression patterns of CsbZIPs in different tissues and under abiotic stresses, 21 CsbZIP genes were differentially expressed between chasmogamous (CH) and cleistogamous (CL) flowers, including two FLOWERING LOCUS D (FD) genes. In shoots and roots, 79.1 and 87.2% of the CsbZIP genes, respectively, displayed transcript changes under at least one stress treatment, such as heat, cold, drought and salt. Strikingly, 17 common CsbZIP genes showed differential expression under stress response and during CL flowering. Co-expression network, GO annotation and real-time quantitative reverse transcription PCR (qRT-PCR) analyses revealed a close relationship between CL flowering-associated genes and abiotic stress-related genes. Conclusions BZIP TFs were comprehensively analysed and identified in allotetraploid C. songorica. Our results provide insights into the evolutionary history of the bZIP family in C. songorica and provide abiotic stress-responsive and CL-associated candidate CsbZIP genes for potential applications in the genetic improvement of plants.
英文关键词	Cleistogenes songorica Transcription factor BZIP genes Evolutionary analysis Cleistogamous Stress response
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000491861300004
WOS关键词	MOLECULAR CHARACTERIZATION ; EXPRESSION ANALYSIS ; DROUGHT TOLERANCE ; ARABIDOPSIS ; GENE ; PROTEIN ; RESISTANCE ; ABA ; EVOLUTIONARY ; SALINITY
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
EI主题词	2019-10-22
来源机构	兰州大学
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310326
作者单位	Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Key Lab Grassland Livestock Ind Innovat, Minist Agr & Rural Affairs,State Key Lab Grasslan, Lanzhou 730020, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Yan, Qi,Wu, Fan,Ma, Tiantian,et al. Comprehensive analysis of bZIP transcription factors uncovers their roles during dimorphic floret differentiation and stress response in Cleistogenes songorica[J]. 兰州大学,2019,20(1).
APA	Yan, Qi.,Wu, Fan.,Ma, Tiantian.,Zong, Xifang.,Ma, Qian.,...&Zhang, Jiyu.(2019).Comprehensive analysis of bZIP transcription factors uncovers their roles during dimorphic floret differentiation and stress response in Cleistogenes songorica.BMC GENOMICS,20(1).
MLA	Yan, Qi,et al."Comprehensive analysis of bZIP transcription factors uncovers their roles during dimorphic floret differentiation and stress response in Cleistogenes songorica".BMC GENOMICS 20.1(2019).