干旱区生态环境知识资源中心(Arid): Targeting the administration of ecdysterone in doping control samples

Arid

DOI	10.1007/s11419-019-00504-y
	Targeting the administration of ecdysterone in doping control samples
	Parr, Maria Kristina 1; Ambrosio, Gabriella 1; Wuest, Bernhard 2; Mazzarino, Monica 3; de la Torre, Xavier 3; Sibilia, Francesca 3; Joseph, Jan Felix 1,4; Diel, Patrick 5; Botre, Francesco 3,6
通讯作者	Parr, Maria Kristina
来源期刊	FORENSIC TOXICOLOGY
ISSN	1860-8965
EISSN	1860-8973
出版年	2020
卷号	38 期号:1 页码:172-184
英文摘要	Purpose The phytosteroid ecdysterone was recently reported to enhance performance in sports and may thus be considered as a substance of relevance in anti-doping control. To trace back an administration of ecdysterone from urine samples, analytical properties have been investigated to assess its integration into initial testing procedures (ITP) in doping control laboratories. Methods Analytical properties of ecdysterone were evaluated using gas chromatography-quadrupole/time-of-flight-mass spectrometry (GC-QTOF-MS) and liquid chromatography (LC)-QTOF-MS. Its metabolism and elimination in human were studied using urines collected after administration. Results The detectability of ecdysterone by GC-MS (after derivatization) and/or LC-MS(/MS) has been demonstrated and sample preparation methods were evaluated. Dilute-and-inject for LC-MS(/MS) or solid phase extraction using Oasis HLB for GC-MS or LC-MS were found most suitable, while liquid-liquid extraction was hampered by the high polarity of ecdysteroids. Most abundantly, ecdysterone was detected in the post administration urines as parent compound besides the metabolite desoxy-ecdysterone. Additionally, desoxy-poststerone was tentatively assigned as minor metabolite, however, further investigations are needed. Conclusion An administration of ecdysterone can be targeted using existing procedures of anti-doping laboratories. Ecdysterone and desoxy-ecdysterone appeared as suitable candidates for integration in ITP. Using dilute-and-inject a detection of the parent compound was possible for more than 2 days after the administration of a single dose of similar to 50 mg.
英文关键词	Doping control Monitoring program Ecdysterone Urine analysis LC-MS/MS GC-MS Accurate mass
类型	Article
语种	英语
国家	Germany ; USA ; Italy
开放获取类型	Green Submitted
收录类别	SCI-E
WOS记录号	WOS:000490193700001
WOS关键词	PROTEIN-SYNTHESIS ; INSECT HORMONES ; 20-HYDROXYECDYSONE ; PHYTOECDYSTEROIDS ; METABOLISM ; ECDISTERONE ; ECDYSONE ; INCREASE ; STEROIDS ; BETA
WOS类目	Toxicology
WOS研究方向	Toxicology
EI主题词	2019-10-15
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310291
作者单位	1.Free Univ Berlin, Inst Pharm, Konigin Luise Str 2 4, D-14195 Berlin, Germany; 2.Agilent Technol, Santa Clara, CA USA; 3.FMSI, Lab Antidoping, Rome, Italy; 4.Free Univ Berlin, Core Facil BioSupraMol, Dept Biol, Chem,Pharm, Berlin, Germany; 5.German Sport Univ Cologne, Inst Cardiovasc Res & Sports Med, Dept Mol & Cellular Sports Med, Cologne, Germany; 6.Sapienza Univ Rome, Dept Expt Med, Rome, Italy
推荐引用方式 GB/T 7714	Parr, Maria Kristina,Ambrosio, Gabriella,Wuest, Bernhard,et al. Targeting the administration of ecdysterone in doping control samples[J],2020,38(1):172-184.
APA	Parr, Maria Kristina.,Ambrosio, Gabriella.,Wuest, Bernhard.,Mazzarino, Monica.,de la Torre, Xavier.,...&Botre, Francesco.(2020).Targeting the administration of ecdysterone in doping control samples.FORENSIC TOXICOLOGY,38(1),172-184.
MLA	Parr, Maria Kristina,et al."Targeting the administration of ecdysterone in doping control samples".FORENSIC TOXICOLOGY 38.1(2020):172-184.