干旱区生态环境知识资源中心(Arid): Over-Expression of UV-Damage DNA Repair Genes and Ribonucleic Acid Persistence Contribute to the Resilience of Dried Biofilms of the Desert Cyanobacetrium Chroococcidiopsis Exposed to Mars-Like UV Flux and Long-Term Desiccation

Arid

DOI	10.3389/fmicb.2019.02312
	Over-Expression of UV-Damage DNA Repair Genes and Ribonucleic Acid Persistence Contribute to the Resilience of Dried Biofilms of the Desert Cyanobacetrium Chroococcidiopsis Exposed to Mars-Like UV Flux and Long-Term Desiccation
	Mosca, Claudia 1; Rothschild, Lynn J.2; Napoli, Alessandro 1; Ferre, Fabrizio 3; Pietrosanto, Marco 1; Fagliarone, Claudia 1; Baque, Mickael 4; Rabbow, Elke 5; Rettberg, Petra 5; Billi, Daniela 1
通讯作者	Billi, Daniela
来源期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
出版年	2019
卷号	10
英文摘要	The survival limits of the desert cyanobacterium Chroococcidiopsis were challenged by rewetting dried biofilms and dried biofilms exposed to 1.5 x 10(3) kJ/m(2) of a Mars-like UV, after 7 years of air-dried storage. PCR-stop assays revealed the presence of DNA lesions in dried biofilms and an increased accumulation in dried-UV-irradiated biofilms. Different types and/or amounts of DNA lesions were highlighted by a different expression of uvrA, uvrB, uvrC, phrA, and uvsE genes in dried-rewetted biofilms and dried-UV-irradiated-rewetted biofilms, after rehydration for 30 and 60 min. The up-regulation in dried-rewetted biofilms of uvsE gene encoding an UV damage endonuclease, suggested that UV-damage DNA repair contributed to the repair of desiccation-induced damage. While the phrA gene encoding a photolyase was up-regulated only in dried-UV-irradiated-rewetted biofilms. Nucleotide excision repair genes were over-expressed in dried-rewetted biofilms and dried-UV-irradiated-rewetted biofilms, with uvrC gene showing the highest increase in dried-UV-irradiated-rewetted biofilms. Dried biofilms preserved intact mRNAs (at least of the investigated genes) and 16S ribosomal RNA that the persistence of the ribosome machinery and mRNAs might have played a key role in the early phase recovery. Results have implications for the search of extra-terrestrial life by contributing to the definition of habitability of astrobiologically relevant targets such as Mars or planets orbiting around other stars.
英文关键词	habitability and astrobiology anhydrobiosis desert cyanobacteria Mars UV simulation DNA repair
类型	Article
语种	英语
国家	Italy ; USA ; Germany
开放获取类型	Green Accepted, gold, Green Published
收录类别	SCI-E
WOS记录号	WOS:000491960000001
WOS关键词	NOSTOC-COMMUNE ; CYANOBACTERIUM CHROOCOCCIDIOPSIS ; TOLERANT CYANOBACTERIUM ; DEINOCOCCUS-RADIODURANS ; OXIDATIVE STRESS ; DORMANT CELLS ; MESSENGER-RNA ; RESISTANCE ; HABITABILITY ; RADIATION
WOS类目	Microbiology
WOS研究方向	Microbiology
EI主题词	2019-10-11
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310273
作者单位	1.Univ Roma Tor Vergata, Dept Biol, Rome, Italy; 2.NASA, Div Earth Sci, Ames Res Ctr, Mountain View, CA USA; 3.Univ Bologna Alma Mater, Dept Pharm & Biotechnol, Bologna, Italy; 4.German Aerosp Ctr, Inst Planetary Res Management & Infrastruct, Astrobiol Labs Res Grp, Berlin, Germany; 5.German Aerosp Ctr, Inst Aerosp Med, Cologne, Germany
推荐引用方式 GB/T 7714	Mosca, Claudia,Rothschild, Lynn J.,Napoli, Alessandro,et al. Over-Expression of UV-Damage DNA Repair Genes and Ribonucleic Acid Persistence Contribute to the Resilience of Dried Biofilms of the Desert Cyanobacetrium Chroococcidiopsis Exposed to Mars-Like UV Flux and Long-Term Desiccation[J],2019,10.
APA	Mosca, Claudia.,Rothschild, Lynn J..,Napoli, Alessandro.,Ferre, Fabrizio.,Pietrosanto, Marco.,...&Billi, Daniela.(2019).Over-Expression of UV-Damage DNA Repair Genes and Ribonucleic Acid Persistence Contribute to the Resilience of Dried Biofilms of the Desert Cyanobacetrium Chroococcidiopsis Exposed to Mars-Like UV Flux and Long-Term Desiccation.FRONTIERS IN MICROBIOLOGY,10.
MLA	Mosca, Claudia,et al."Over-Expression of UV-Damage DNA Repair Genes and Ribonucleic Acid Persistence Contribute to the Resilience of Dried Biofilms of the Desert Cyanobacetrium Chroococcidiopsis Exposed to Mars-Like UV Flux and Long-Term Desiccation".FRONTIERS IN MICROBIOLOGY 10(2019).