干旱区生态环境知识资源中心(Arid): A high throughput method for screening deep-seeding tolerance in sorghum

Arid

DOI	10.1007/s10722-019-00835-0
	A high throughput method for screening deep-seeding tolerance in sorghum
	Zou, Guihua 1; Zhou, Lengbo 2; Zhai, Guowei 1; Ding, Yanqing 2; Lu, Ping 3; Liu, Heqin 1; Zhen, Xueqiang 1; Liu, Xiuhui 1; Zhang, Liyi 2; Xin, Zhanguo 4; Chen, Heyun 1
通讯作者	Chen, Heyun
来源期刊	GENETIC RESOURCES AND CROP EVOLUTION
ISSN	0925-9864
EISSN	1573-5109
出版年	2019
卷号	66 期号:8 页码:1643-1651
英文摘要	Low soil moisture is a major abiotic stress wide spread in arid and semi-arid regions, limiting both seed germination and seedling establishment. Deep-seeding technique allows seeds to use the moisture in deep soil, however, most sorghum seeds cannot penetrate soil layer of 10 cm or deeper. To develop sorghum varieties tolerant to deep-seeding, it is necessary to identify promising accessions with good agronomic traits and tolerance to deep-seeding. A high throughput hydroponic method was developed to screen sorghum accessions with long mesocotyl under dark conditions. This method revealed large genetic variations for mesocotyl length in the panel of 105 sorghum accessions, which were validated by soil culture and deep-seeding experiments. The lines with long mesocotyl under hydroponic culture had good seedling establishments in deep-seeding experiments, while the lines with short mesocotyl length could not emerge. Compared to conventional sand or soil cultural methods, the hydroponic culture was highly efficient that requires less time, space, and labor. The mesocotyl lengths of screened sorghum accessions under the newly developed hydroponic method were highly correlated with soil culture and germination rate under deep-seeding in soil. As an efficient screening tool for the mesocotyl lengths under soil culture and the seedling emergence rates from deep soil layer, the hydroponic culture method can be easily integrated in breeding programs targeting deep-seeding tolerance. Breeding for long mesocotyl sorghum varieties may lead to good seedling establishments under arid and semi-arid regions.
英文关键词	Deep-seeding tolerance High throughput screening method Mesocotyl elongation Sorghum
类型	Article
语种	英语
国家	Peoples R China ; USA
收录类别	SCI-E
WOS记录号	WOS:000492473000002
WOS关键词	MESOCOTYL ELONGATION ; SEEDLING EMERGENCE ; COLEOPTILE LENGTH ; INTERNODE ELONGATION ; GENETIC-ANALYSIS ; 1ST INTERNODE ; RICE ; WHEAT ; ETHYLENE ; CHARACTERS
WOS类目	Agronomy ; Plant Sciences
WOS研究方向	Agriculture ; Plant Sciences
EI主题词	2019-10-11
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310270
作者单位	1.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou 310021, Zhejiang, Peoples R China; 2.Guizhou Acad Agr Sci, Inst Upland Food Crops, Guiyang 550009, Guizhou, Peoples R China; 3.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China; 4.USDA ARS, Plant Stress & Germplasm Dev Unit, Cropping Syst Res Lab, Lubbock, TX 79415 USA
推荐引用方式 GB/T 7714	Zou, Guihua,Zhou, Lengbo,Zhai, Guowei,et al. A high throughput method for screening deep-seeding tolerance in sorghum[J],2019,66(8):1643-1651.
APA	Zou, Guihua.,Zhou, Lengbo.,Zhai, Guowei.,Ding, Yanqing.,Lu, Ping.,...&Chen, Heyun.(2019).A high throughput method for screening deep-seeding tolerance in sorghum.GENETIC RESOURCES AND CROP EVOLUTION,66(8),1643-1651.
MLA	Zou, Guihua,et al."A high throughput method for screening deep-seeding tolerance in sorghum".GENETIC RESOURCES AND CROP EVOLUTION 66.8(2019):1643-1651.