干旱区生态环境知识资源中心(Arid): Constitutive expression of chloroplast glycerol-3-phosphate acyltransferase from Ammopiptanthus mongolicus enhances unsaturation of chloroplast lipids and tolerance to chilling, freezing and oxidative stress in transgenic Arabidopsis

Arid

DOI	10.1016/j.plaphy.2019.07.019
	Constitutive expression of chloroplast glycerol-3-phosphate acyltransferase from Ammopiptanthus mongolicus enhances unsaturation of chloroplast lipids and tolerance to chilling, freezing and oxidative stress in transgenic Arabidopsis
	Xue, Min; Guo, Ting; Ren, Meiyan; Wang, Zhilin; Tang, Kuangang; Zhang, Wenjun; Wang, Maoyan
通讯作者	Wang, Maoyan
来源期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
出版年	2019
卷号	143 页码:375-387
英文摘要	Chloroplast glycerol-3-phosphate acyltransferase (GPAT) is the first key enzyme determining the unsaturation of phosphatidylglycerol (PG) in thylakoid membranes and is involved in the tolerance of plants to chilling, heat and high salinity. However, whether the GPAT affects plant tolerance to other stressors has been scarcely reported. Ammopiptanthus mongolicus is the only evergreen broadleaf shrub growing in the central Asian desert, and it has a high tolerance to harsh environments, especially extreme cold. This study aimed to characterize the physiological function of AmGPAT from A. mongolicus. The transcription of AmGPAT was markedly induced by cold and drought but differentially suppressed by heat and high salinity in the laboratory-cultured seedlings. The gene also had the highest transcription levels in the leaves of shrubs naturally growing in the wild during the late autumn and winter months throughout the year. Moreover, AmGPAT was most abundantly expressed in leaves and immature pods rather than other organs of the shrubs. Constitutive expression of AmGPAT in Arabidopsis increased the levels of cis-unsaturated fatty acids, especially that of linolenic acid (18:3), mainly in PG but also in other chloroplast lipids in transgenic lines. More importantly, the transgene significantly increased the tolerance of the transgenics not only to chilling but also to freezing and oxidative stress at both the cellular and wholeplant levels. In contrast, this gene reduced heat tolerance of the transgenic plants. This study improves the current understanding of chloroplast GPAT in plant tolerance against abiotic stressors through regulating the unsaturation of chloroplast lipids, mainly that of PG.
英文关键词	Ammopiptanthus mongolicus Glycerol-3-phosphate acyltransferase Phosphatidylglycerol Temperature stress Oxidative stress
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000490633100036
WOS关键词	FATTY-ACID UNSATURATION ; LOW-TEMPERATURE ; GENE-EXPRESSION ; PHOSPHATIDYLGLYCEROL ; PHOTOINHIBITION ; INCREASE ; OVEREXPRESSION ; PHOTOSYNTHESIS ; MACHINERY ; IMPROVES
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
EI主题词	2019-10-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310224
作者单位	Inner Mongolia Agr Univ, Coll Life Sci, 306 Zhaowuda St, Hohhot 010018, Peoples R China
推荐引用方式 GB/T 7714	Xue, Min,Guo, Ting,Ren, Meiyan,et al. Constitutive expression of chloroplast glycerol-3-phosphate acyltransferase from Ammopiptanthus mongolicus enhances unsaturation of chloroplast lipids and tolerance to chilling, freezing and oxidative stress in transgenic Arabidopsis[J],2019,143:375-387.
APA	Xue, Min.,Guo, Ting.,Ren, Meiyan.,Wang, Zhilin.,Tang, Kuangang.,...&Wang, Maoyan.(2019).Constitutive expression of chloroplast glycerol-3-phosphate acyltransferase from Ammopiptanthus mongolicus enhances unsaturation of chloroplast lipids and tolerance to chilling, freezing and oxidative stress in transgenic Arabidopsis.PLANT PHYSIOLOGY AND BIOCHEMISTRY,143,375-387.
MLA	Xue, Min,et al."Constitutive expression of chloroplast glycerol-3-phosphate acyltransferase from Ammopiptanthus mongolicus enhances unsaturation of chloroplast lipids and tolerance to chilling, freezing and oxidative stress in transgenic Arabidopsis".PLANT PHYSIOLOGY AND BIOCHEMISTRY 143(2019):375-387.