干旱区生态环境知识资源中心(Arid): Functional identification of Ammopiptanthus mongolicus anion channel AmSLAC1 involved in drought induced stomata closure

Arid

DOI	10.1016/j.plaphy.2019.09.012
	Functional identification of Ammopiptanthus mongolicus anion channel AmSLAC1 involved in drought induced stomata closure
	Li Junlin 1,4; Han Lei 2,3; Su Yanhua 3; Guo Hongen 1; Zhang Huanchao 4,5
通讯作者	Zhang Huanchao
来源期刊	PLANT PHYSIOLOGY AND BIOCHEMISTRY
ISSN	0981-9428
出版年	2019
卷号	143 页码:340-350
英文摘要	Drought, one of the most acute abiotic stressors plants encountered, can adversely affect plants growth and development. The fast adjustment of stomatal aperture is necessary for effective drought tolerance in plants. Anion channels were identified as important controllers of stomatal closing via mediating anion efflux. The present study reports the isolation and identification of a SLAC (SLOW ANION CHANNEL-ASSOCIATED 1) or-tholog from an ancient desert shrub Ammopiptanthus mongolicus (Maxim.) Cheng f. (AmSLAC1), which is functionally conserved for ABA and drought induced stomata closure. AmSLACI was primarily expressed in shoots, especially in guard cells. The transcription of AmSLAC1 was induced in response to ABA and PEG treatments, implying the potential involvement in ABA-induced drought stress responses. Fluorescence observation suggested that AmSLAC1 was localized in the plasma membrane. BiFC asssays demonstrated an interaction between AmSLAC1 and the typical calcium-dependent protein kinases AmCPK6. Ectopic expression of AmSLAC1 restores a slac1-defective phenotype in Arabidopsis. Furthermore, anion conductance mediated by AmSLAC1 can be activated by AmCPK6 in Xenopus oocytes. Taken together, these results demonstrate that the expression of AmSLAC1 enables the complement of the phenotypes of Arabidopsis slac1 mutants, indicating that AmSLAC1, as an anion channel and regulated by AmCPK6, is functionally conserved for ABA and drought induced stomata closure.
英文关键词	S-type anion channel Ammopiptanthus mongolicus Stomatal closure Drought
类型	Article
语种	英语
国家	Peoples R China
收录类别	SCI-E
WOS记录号	WOS:000490633100033
WOS关键词	GUARD-CELL ; SLAC1 ; KINASES ; GENE ; CA2+
WOS类目	Plant Sciences
WOS研究方向	Plant Sciences
EI主题词	2019-10-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310222
作者单位	1.Shandong Inst Sericulture, Yantai 264002, Peoples R China; 2.Lu Dong Univ, Yantai 264025, Peoples R China; 3.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu, Peoples R China; 4.Nanjing Forestry Univ, Coll Forestry, Nanjing 210037, Jiangsu, Peoples R China; 5.Nanjing Forest Univ, Coinnovat Ctr Sustainable Forestry Southern China, Nanjing 210037, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Li Junlin,Han Lei,Su Yanhua,et al. Functional identification of Ammopiptanthus mongolicus anion channel AmSLAC1 involved in drought induced stomata closure[J],2019,143:340-350.
APA	Li Junlin,Han Lei,Su Yanhua,Guo Hongen,&Zhang Huanchao.(2019).Functional identification of Ammopiptanthus mongolicus anion channel AmSLAC1 involved in drought induced stomata closure.PLANT PHYSIOLOGY AND BIOCHEMISTRY,143,340-350.
MLA	Li Junlin,et al."Functional identification of Ammopiptanthus mongolicus anion channel AmSLAC1 involved in drought induced stomata closure".PLANT PHYSIOLOGY AND BIOCHEMISTRY 143(2019):340-350.