干旱区生态环境知识资源中心(Arid): Bioguided Purification of Active Compounds from Leaves of Anadenanthera colubrina var. cebil (Griseb.) Altschul

Arid

DOI	10.3390/biom9100590
	Bioguided Purification of Active Compounds from Leaves of Anadenanthera colubrina var. cebil (Griseb.) Altschul
	Cavalcante de Araujo, Daniel Rodrigo 1,2; da Silva, Tulio Diego 3; Harand, Wolfgang 1; de Andrade Lima, Claudia Sampaio 4; Ferreira Neto, Joao Paulo 4; Ramos, Barbara de Azevedo 2; Rocha, Tamiris Alves 2; Alves, Harley da Silva 5; de Sousa, Rayane Sobrinho 6; de Oliveira, Ana Paula 7; Nascimento da Silva, Luis Claudio 6; Guedes da Silva Almeida, Jackson Roberto 7; da Silva, Marcia Vanusa 2; dos Santos Correia, Maria Tereza 2
通讯作者	Cavalcante de Araujo, Daniel Rodrigo ; Nascimento da Silva, Luis Claudio
来源期刊	BIOMOLECULES
EISSN	2218-273X
出版年	2019
卷号	9 期号:10
英文摘要	Anadenanthera colubrina var cebil (Griseb.) Altschul is a medicinal plant found throughout the Brazilian semi-arid area. This work performed a bioguided purification of active substances present in ethyl acetate extract from A. colubrina leaves. The anti-Staphylococcus aureus and antioxidant actions were used as markers of bioactivity. The extract was subjected to flash chromatography resulting in five fractions (F1, F2, F3, F4, and F5). The fractions F2 and F4 presented the highest antimicrobial action, with a dose able to inhibit 50% of bacteria growth (IN50) of 19.53 mu g/mL for S. aureus UFPEDA 02; whereas F4 showed higher inhibitory action towards DPPH radical (2,2-diphenyl-1-picryl-hydrazyl-hydrate) [dose able to inhibit 50% of the radical (IC50) = 133 +/- 9 mu g/mL]. F2 and F4 were then subjected to preparative high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR), resulting in the identification of p-hydroxybenzoic acid and hyperoside as the major compounds in F2 and F4, respectively. Hyperoside and p-hydroxybenzoic acid presented IN50 values of 250 mu g/mL and 500 mu g/mL against S. aureus UFPEDA 02, respectively. However, the hyperoside had an IN50 of 62.5 mu g/mL against S. aureus UFPEDA 705, a clinical isolate with multidrug resistant phenotype. Among the purified compounds, the proanthocyanidins obtained from F2 exhibited the higher antioxidant potentials. Taken together, these results highlight the potential of A. colubrina leaves as an alternative source of biomolecules of interest for the pharmaceutical, food, and cosmetic industries.
英文关键词	Staphylococcus aureus medicinal plant compound purification
类型	Article
语种	英语
国家	Brazil
开放获取类型	Green Published, gold
收录类别	SCI-E
WOS记录号	WOS:000497726800082
WOS关键词	4-HYDROXYBENZOIC ACID ; OXIDATIVE STRESS ; ANTIMICROBIAL ACTIVITY ; STAPHYLOCOCCUS-AUREUS ; ANTIOXIDANT CAPACITY ; PHENOLIC-COMPOUNDS ; HYPEROSIDE ; EXTRACT ; PLANTS ; MECHANISM
WOS类目	Biochemistry & Molecular Biology
WOS研究方向	Biochemistry & Molecular Biology
EI主题词	2019-10-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310146
作者单位	1.Inst Nacl Semiarido INSA MCTIC, BR-58429500 Campina Grande, Paraiba, Brazil; 2.Univ Fed Pernambuco UFPE, Ctr Biociencias, Dept Bioquim, BR-50670901 Recife, PE, Brazil; 3.Ctr Tecnol Estrateg Nordeste CETENE MCTIC, BR-50670901 Recife, PE, Brazil; 4.Univ Fed Pernambuco UFPE, Ctr Biociencias, Dept Biofis & Radiobiol, BR-50670901 Recife, PE, Brazil; 5.Univ Estadual Paraiba UEPB, Dept Farm & Posgrad Ciencias Farmaceut, Lab Fitoquim, BR-58429500 Campina Grande, Paraiba, Brazil; 6.Univ Ceuma, Lab Patogenicidade Microbiana, BR-65080805 Sao Luis, Maranhao, Brazil; 7.Univ Fed Vale Sao Francisco CAFMA UNIVASF, Cent Anal Farmacos Medicamentos & Alimentos, BR-56328903 Petrolina, Brazil
推荐引用方式 GB/T 7714	Cavalcante de Araujo, Daniel Rodrigo,da Silva, Tulio Diego,Harand, Wolfgang,et al. Bioguided Purification of Active Compounds from Leaves of Anadenanthera colubrina var. cebil (Griseb.) Altschul[J],2019,9(10).
APA	Cavalcante de Araujo, Daniel Rodrigo.,da Silva, Tulio Diego.,Harand, Wolfgang.,de Andrade Lima, Claudia Sampaio.,Ferreira Neto, Joao Paulo.,...&dos Santos Correia, Maria Tereza.(2019).Bioguided Purification of Active Compounds from Leaves of Anadenanthera colubrina var. cebil (Griseb.) Altschul.BIOMOLECULES,9(10).
MLA	Cavalcante de Araujo, Daniel Rodrigo,et al."Bioguided Purification of Active Compounds from Leaves of Anadenanthera colubrina var. cebil (Griseb.) Altschul".BIOMOLECULES 9.10(2019).