干旱区生态环境知识资源中心(Arid): Functional Analysis of Three miRNAs in Agropyron mongolicum Keng under Drought Stress

Arid

DOI	10.3390/agronomy9100661
	Functional Analysis of Three miRNAs in Agropyron mongolicum Keng under Drought Stress
	Zhang, Xuting 1; Fan, Bobo 1; Yu, Zhuo 1; Nie, Lizhen 2; Zhao, Yan 3; Yu, Xiaoxia 1; Sun, Fengcheng 2; Lei, Xuefeng 1; Ma, Yanhong 1
通讯作者	Ma, Yanhong
来源期刊	AGRONOMY-BASEL
EISSN	2073-4395
出版年	2019
卷号	9 期号:10
英文摘要	Agropyron mongolicum Keng, a perennial diploid grass with high drought tolerance, belongs to the genus Agropyron, tribe Triticeae. It has made tremendous contributions toward reseeding natural pasture and seeding artificial grassland in China, especially in the arid and semi-arid area of northern China. As a wild relative of wheat, A. mongolicum is also a valuable resource for the genetic improvement of wheat crops. MicroRNAs are small non-coding RNA molecules ubiquitous in plants, which have been involved in responses to a wide variety of stresses including drought, salinity, chilling temperature. To date, little research has been done on drought-responsive miRNAs in A. mongolicum. In this study, two miRNA libraries of A. mongolicum under drought and normal conditions were constructed, and drought-responsive miRNAs were screened via Solexa high throughput sequencing and bioinformatic analysis. A total of 114 new miRNAs were identified in A. mongolicum including 53 conservative and 61 unconservative miRNAs, and 1393 target genes of 98 miRNAs were predicted. Seventeen miRNAs were found to be differentially expressed under drought stress, seven (amo-miR21, amo-miR62, amo-miR82, amo-miR5, amo-miR77, amo-miR44 and amo-miR17) of which were predicted to target on genes involved in drought tolerance. QRT-PCR analysis confirmed the expression changes of the seven drought related miRNAs in A. mongolicum. We then transformed the seven miRNAs into Arabidopsis thaliana plants, and three of them (amo-miR21, amo-miR5 and amo-miR62) were genetically stable. The three miRNAs demonstrated the same expression pattern in A. thaliana as that in A. mongolicum under drought stress. Findings from this study will better our understanding of the molecular mechanism of miRNAs in drought tolerance and promote molecular breeding of forage grass with improved adaption to drought.
英文关键词	Agropyron mongolicum Keng drought-responsive microRNA NEXT-generation sequencing target gene prediction functional analysis
类型	Article
语种	英语
国家	Peoples R China
开放获取类型	gold
收录类别	SCI-E
WOS记录号	WOS:000498268600095
WOS关键词	FLORAL DIP TRANSFORMATION ; TRANSCRIPTION FACTOR ; GENOME-WIDE ; MICRORNAS ; RNA ; IDENTIFICATION ; BIOGENESIS ; CLONING ; FAMILY ; GENES
WOS类目	Agronomy ; Plant Sciences
WOS研究方向	Agriculture ; Plant Sciences
EI主题词	2019-10-01
资源类型	期刊论文
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/310139
作者单位	1.Inner Mongolia Agr Univ, Agr Coll, Hohhot 010000, Peoples R China; 2.Inner Mongolia Acad Agr & Anim Husb Sci, Hohhot 010000, Peoples R China; 3.Inner Mongolia Agr Univ, Coll Grassland Resources & Environm, Hohhot 010000, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xuting,Fan, Bobo,Yu, Zhuo,et al. Functional Analysis of Three miRNAs in Agropyron mongolicum Keng under Drought Stress[J],2019,9(10).
APA	Zhang, Xuting.,Fan, Bobo.,Yu, Zhuo.,Nie, Lizhen.,Zhao, Yan.,...&Ma, Yanhong.(2019).Functional Analysis of Three miRNAs in Agropyron mongolicum Keng under Drought Stress.AGRONOMY-BASEL,9(10).
MLA	Zhang, Xuting,et al."Functional Analysis of Three miRNAs in Agropyron mongolicum Keng under Drought Stress".AGRONOMY-BASEL 9.10(2019).