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Nowadays, the desert vegetation is becoming more under threat due to drought and other conditions, such as low level of rain, dry soil and high temperature fluctuations. Aerva javanica (Burm. F) Juss ex Schult. is a well-known species by the Saharan population for its effectiveness against snake bites and scorpion stings, which is supported also by the previous ethno botanical survey. It is a dioecious plant, widely reported to have a greater distribution of male than female plants in Africa, however the female plants are the mostly used ones by the native population. Nevertheless, little has been done to multiply this plant by in vitro culture to this day. Establishing an efficient regeneration system through callus production is necessary for its conservation, as well as for isolation of bioactive molecules used in folk medicine. Present study investigated the frequency of callus formation from this plant material under different culture conditions. Several assays of growth regulator combinations showed an efficient callus regeneration system and subsequent plant expression. The Murashige and Skoog medium containing 22.2 mu M 6-benzyladenine (6-BA) was optimal for callus initiation. Its combination with indole acetic acid (IAA) has provided callus masses with bud induction.