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Desertification is becoming a global problem and use of soil amendments is one of the options to decrease the danger. Many authors indicate that biochar can affect soil hydro-limits in a positive way. Unfortunately biochar also affects biological properties of soil. Predictions of the effect of biochar application are very difficult and must be based on aromatic substance concentration and on the dosage of biochar. In our experiment we prepared pots for establishing the effect on different substrates. Each container contained two separate parts with two different substrates. We measured the concentration of base groups of microorganisms on Lactuca sativa roots and the decomposition of cellulose. According to our results biochar can mitigate the impact of stress caused by drought on soil microorganisms. These amendments did not affect cellulose decomposition.