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MODIS (Moderate Resolution Imaging Spectroradiometer) is a key sensor aboard the Terra (EOS AM) and Aqua (EOS PM) satellites. MODIS collects data in 36 spectral bands and generates over 40 data products for land, atmosphere, cryosphere and oceans. MODIS bands 1 and 2 have nadir spatial resolution of 250 m, compared with 500 m for bands 3 to 7 and 1000 m for all the remaining bands, and their measurements are crucial to derive key land surface products. This study evaluates the calibration performance of the Collection-6 L1B for both Terra and Aqua MODIS bands 1 and 2 using three vicarious approaches. The first and second approaches focus on stability assessment using data collected from two pseudo-invariant sites, Libya 4 desert and Antarctic Dome C snow surface. The third approach examines the relative stability between Terra and Aqua in reference to a third sensor from a series of NOAA 15-19 Advanced Very High Resolution Radiometer (AVHRR). The comparison is based on measurements from MODIS and AVHRR Simultaneous Nadir Overpasses (SNO) over a thirteen-year period from 2002 to 2015. Results from this study provide a quantitative assessment of Terra and Aqua MODIS bands 1 and 2 calibration stability and the relative calibration differences between the two sensors.