Knowledge Resource Center for Ecological Environment in Arid Area
DOI | 10.4028/www.scientific.net/AMR.726-731.3680 |
Denaturing Gradient Gel Electrophoresis Detects Bacterial and Cyaonbacterial Diversities in Biological Soil Crusts in a Semiarid Desert, China | |
Zhang, Ying; Cao, Chengyou; Zhang, Peng | |
通讯作者 | Cao, Chengyou |
会议名称 | 2nd International Conference on Energy and Environmental Protection (ICEEP 2013) |
会议日期 | APR 19-21, 2013 |
会议地点 | Guilin, PEOPLES R CHINA |
英文摘要 | The purpose of this study is to assess the application of denaturing gradient gel electrophoresis (DGGE) for analyzing the bacterial and cyanobacterial diversities of biological soil crusts (BSCs) in sandy land. Soil microbial DNA was extracted from BSCs under different plantations in Horqin Sandy Land of Northeast China. 16S rRNA gene fragments from bacteria and cyanobacteria were amplified by universal bacterial and cyanobacteria-specific primers. Fourteen and six prominent bands were detected in the bacterial and cyanobacterial DGGE profiles, respectively. These bands were excised, cloned and sequenced. Phylogenetic analysis classified the bacterial sequences into the following main groups: Escherichia, Bacillus, Paenibacillus, Shigella, and Pseudomonas. The cyanobacterial sequences were classified as Microcoleus, Leptolyngbya and Haslea. Our study suggests that DGGE is a useful technique for detecting dominant species compositions of bacterial and cyanobacterial communities in biological soil crusts, and specific primers are recommended for PCR of 16S rRNA gene fragments. |
英文关键词 | Denaturing gradient gel electrophoresis diversity Bacteria Cyanobacteria Biological soil crust |
来源出版物 | ADVANCES IN ENVIRONMENTAL TECHNOLOGIES, PTS 1-6 |
ISSN | 1022-6680 |
出版年 | 2013 |
卷号 | 726-731 |
页码 | 3680-3684 |
ISBN | 978-3-03785-742-7 |
出版者 | TRANS TECH PUBLICATIONS LTD |
类型 | Proceedings Paper |
语种 | 英语 |
国家 | Peoples R China |
收录类别 | CPCI-S |
WOS记录号 | WOS:000333767501227 |
WOS类目 | Engineering, Environmental ; Environmental Sciences ; Materials Science, Multidisciplinary |
WOS研究方向 | Engineering ; Environmental Sciences & Ecology ; Materials Science |
资源类型 | 会议论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/301798 |
作者单位 | Northeastern Univ, Coll Sci, Shenyang 110004, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Ying,Cao, Chengyou,Zhang, Peng. Denaturing Gradient Gel Electrophoresis Detects Bacterial and Cyaonbacterial Diversities in Biological Soil Crusts in a Semiarid Desert, China[C]:TRANS TECH PUBLICATIONS LTD,2013:3680-3684. |
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