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Desertification reversal is actually the change of shifting sand to more typical zonal soil. During the reversal process, soil properties has drastically changed, and assessment of these changes is essential for soil management and soil health. The activities of urease, alkaline phosphatase, invertase and catalase, soil organic matter, total nitrogen, phosphorus and potassium, available nitrogen, phosphorus and potassium, cation exchange capacity (CEC) contents of soils during reversal of desertification were investigated, and multivariate analysis was used to study the relationship between soil enzymes and soil physical-chemical properties. The results showed that urease activity, alkaline phosphatise activity and invertase activity were significantly higher from fixed sand land to semi-fixed sand land to semi mobile-sand land to mobile sand land. Soil organic matter had significant positive correlation with activities of invertase, urease and alkaline phosphatase. Soil organic matter, total N and available N were the major factors influencing activities of soil enzymes.