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Iran is an important area for the gene pool of almonds. It has been suggested that Amygdalus communis spread from Iran, Caucasia and the South of Turkey to Syria, Lebanon and Jordan. So far, 21 wild species and 7 inter-species hybrids have been identified in Iran, of which ten are supposed endemic species. Wild almond species have been used traditionally as rootstocks in some areas of Iran. Scions of A. spartioiedes and A. spinosissima are grafted by local almond growers in arid regions onto A. scoparia, A. eriocalda, A. horrida and A. elaengifolia species used as rootstocks. Seedlings of eight almond species have been evaluated for investigating the establishment and vigor of 14 ecotypes from four provinces. The germinated seeds were organized in a completely randomized design with four replicates. Seedlings were planted in plastic bags in outdoor conditions. Seedling establishment and growth parameters were recorded during the growing season. Two ecotypes of A. reticulata had the highest growth and highest stem length at all growth stages.