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Development and aplication of mu Elution plate for the extraction of phenolic compounds from sea algae and their analysis using RRLC/MS/MS is described. The extraction and identification of phenolic compounds is presented from five different sea algae samples, two brown algae (Cystoseira abies + Cystoseira abies dried with nitrogen, Undaria pinnatifida) and two red algae (Sargassum muticum, Chondrus crispus) via solid phase extraction (SPE) using Oasis mu Elution plate. Selected groups of benzoic acid derivatives (p-hydroxybenzoic, protocatechuic, gallic, vanillic and syringic acid), hydroxybenzaldehydes (4-hydroxybenzaldehyde and 3,4-dihydroxybenzaldehyde) and cinnamic acid derivatives (o-coumaric, p-coumaric, caffeic, ferulic, sinapic and chlorogenic acid) were investigated.

Recoveries in range 96-100% were obtained, with LOQs 0.01-2.1 pg/inj and LODs 0.03-7.1 pg/inj, i.e. in the range of low mu m. The SPE enabled to avoid the evaporation step and preconcentrate the analytes directly. The applied method allowed a simultaneous determination of phenols in less than 5 minutes. Thus, the analysis of different plant species containing trace amounts of polar phenols became possible.

Phenolic compounds contained in algae were extracted using Oasis mu Elution plate for its increased sensitivity and pre-concentration effect - extract volume is only 500 mu l. Robotic mechanism of the HPLC instrument is able to use the mu Elution plate trapping part directly and take samples from it.