Knowledge Resource Center for Ecological Environment in Arid Area
Investigation of Somatic Embryogenesis for In Vitro Cultures of Date Palm (Phoenix dactylifera L.) | |
Asemota, O.; Eke, C. R.; Emoghene, B. O.; Aisueni, N. O.; Adetunji, O. A.; Gidado, R. M.; Solomon, B. O. | |
通讯作者 | Asemota, O. |
会议名称 | 4th International Date Palm Conference |
会议日期 | MAR 15-17, 2010 |
会议地点 | Abu Dhabi, U ARAB EMIRATES |
英文摘要 | Date palm is increasingly recognised as an important crop in northern Nigeria. Agriculturally, it can sustainably generate incomes for farmers to take families out of the poverty line while at the same time it can be used as a tree crop to check desertification. To achieve these expectations, a national date palm development program involving planting material production and date palm agronomy is currently underway. The somatic embryogenesis method is being currently adopted to produce planting materials. Apical meristem and leaf explants were used to initiate in vitro cultures. A range of media and media conditions were tested and different media induced callus. These media were supplemented with different growth regulators, sucrose at different concentrations and nitrogen in the growth medium. NAA and 2,4-D provoked callus production. Callus could be produced at various sucrose concentration levels but 30 g/L was optimum. Callus generation potential was best from apical meristems, followed by leaf bases and leaves. Similarly different media also induced somatic embryos but the most reliable medium was the one containing 0.05 mg/L NAA and 1 mg/L 2-ip. The somatic embryos readily developed into shoots which could be rooted in NAA and sucrose (45-90 g/L). Some plants are in the nursery while some have been planted in the field undergoing observation. |
英文关键词 | Phoenix dactylifera in vitro culture growth regulators sucrose |
来源出版物 | IV INTERNATIONAL DATE PALM CONFERENCE |
ISSN | 0567-7572 |
EISSN | 2406-6168 |
出版年 | 2010 |
卷号 | 882 |
页码 | 225-231 |
EISBN | 978-90-6605-633-6 |
出版者 | INT SOC HORTICULTURAL SCIENCE |
类型 | Proceedings Paper |
语种 | 英语 |
国家 | Nigeria |
收录类别 | CPCI-S |
WOS记录号 | WOS:000323618500025 |
WOS关键词 | PROPAGATION ; NITROGEN ; GROWTH |
WOS类目 | Agronomy ; Horticulture |
WOS研究方向 | Agriculture |
资源类型 | 会议论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/298345 |
作者单位 | Nigerian Inst Oil Palm Res, Benin, Edo State, Nigeria |
推荐引用方式 GB/T 7714 | Asemota, O.,Eke, C. R.,Emoghene, B. O.,et al. Investigation of Somatic Embryogenesis for In Vitro Cultures of Date Palm (Phoenix dactylifera L.)[C]:INT SOC HORTICULTURAL SCIENCE,2010:225-231. |
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