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The Sambhar salt lake is the largest single salt source situated in Rajasthan state which experiences desert conditions. It is situated in latitude 26 degrees 58' N and longitude 75 degrees 5' E on the east of the Aravalli hills. The lake bed varies from 1181 feet to 1196.76 feet above the sea level. The initial source of brine in the lake is catchment water about 12-13 inches of rainfall annually mostly during April to September which results in the accumulation of about 35 inches depth of water in the lake in normal year. The density water is less than 1 degrees Be which rises to about 3 Be during September. This water is pumped into the main reservoir and the salinity increases 6-8 degrees Be and later this is pumped into condenses and further density increases to 22-24 degrees Be. During the process micro algae appear and impart different colors. At higher concentrations micro algae are destroyed. The temperature varied between 7.12 (December) to 44.5(June) 2005-2006. The humidity was highest in July '05, 81.49% when the rainfall was also maximum 204.5 mm. Low percentage of humidity of 37.34% was recorded in May '05 when there is no rainfall which corresponds with high temperature in May 43.28 degrees c. The total rainfall for the year 2005-2006 was 391.0 mm out of which the maximum rain of 204.5mm was recorded in July'05. The sodium chloride content available in the subsoil brine at 16 Be,17 Be and 21 degrees Be are 82%, 81.74% and 78.82% respectively. However in the lake brine at 3 degrees Be NaCl was reported as 88.74%. The bacterial load in the different brine samples were analysed by dilution plating technique which shows higher concentration of 1000 cfu/ml in 100 ppm salts. The study indicated the isolates belong to Bacillus and Halobacterium. The samples indicated the percentage of occurrence and the presence of several species of saltern bacteria such as Bacillus subtilis, B. amyloliquefaciens, B. sphaericus, B. licheniformis, Halobacterium sp;Micrococcus sp and Staphylococcus capitis. It was observed that brine algae Dunaliella salina blooms making the brine red in the crystallisers. It is assumed that Dunaliella population in the hypersaline crystallizers of 24-28 degrees Be. supplies the organic compounds required for the growth of halophilic archaea and probably the glycerol is the most important nutrient available to the halobacterial community. Taking advantage of the role of Anemia, there is ample scope for mass management of extensive area of salt work for biological management in a balanced way. This will result in quality and quantity of production of salt and its by products.