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DOI | 10.1016/S0021-9673(01)01438-8 |
Determination of reboxetine, a recent antidepressant drug, in human plasma by means of two high-performance liquid chromatography methods | |
Raggi, MA; Mandrioli, R; Casamenti, G; Volterra, V; Pinzauti, S | |
通讯作者 | Raggi, MA |
会议名称 | 25th International Symposium on High Performance Liquid Phase Separations and Related Techniques |
会议日期 | JUN 17-22, 2001 |
会议地点 | MAASTRICHT, NETHERLANDS |
英文摘要 | Reboxetine is a new norepinephrine reuptake inhibitor (NRI) drug recently introduced in the therapy for depressed patients. It is effective in the treatment of severe depression and safer to use than traditional tricyclic antidepressants. In this paper an original high-performance liquid chromatography (HPLC) method with ultraviolet detection for the determination of reboxetine in human plasma is described. It uses a C,, reversed-phase column and a mobile phase composed of acetonitrile and aqueous tetramethylammonium perchlorate. For the analysis of plasma samples containing very low levels of reboxetine, another HPLC method with fluorimetric detection was developed (limit of quantitation, LOQ=11 ng ml(-1); limit of detection, LOD=4 ng ml(-1)). The fluorimetric method is based on precolumn derivatisation of reboxedne with 9-fluorenylmethyl chloroformate. An accurate sample pretreatment of human plasma samples has been implemented by means of solid-phase extraction (SPE) on Oasis HLB (hydrophilic-lipophilic balance) cartridges with very high extraction yields (>95%). Both methods were applied to the analysis of plasma samples from depressed patients undergoing therapy with reboxetine and gave satisfactory results in terms of precision (RSD<4.5%) and accuracy (mean recovery>94%). (C) 2002 Elsevier Science B.V. All rights reserved. |
英文关键词 | pharmaceutical analysis UV detection fluorimetric detection human plasma reboxetine |
来源出版物 | JOURNAL OF CHROMATOGRAPHY A |
ISSN | 0021-9673 |
出版年 | 2002 |
卷号 | 949 |
期号 | 1-2 |
页码 | 23-33 |
出版者 | ELSEVIER SCIENCE BV |
类型 | Article;Proceedings Paper |
语种 | 英语 |
国家 | Italy |
收录类别 | CPCI-S ; SCI-E |
WOS记录号 | WOS:000174633300003 |
WOS关键词 | CHIRAL DERIVATIZATION ; ENANTIOMERS ; PHARMACOKINETICS ; DEPRESSION ; RAT |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
资源类型 | 会议论文 |
条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/293867 |
作者单位 | (1)Univ Bologna, Dept Pharmaceut Sci, I-40126 Bologna, Italy;(2)Univ Bologna, Psychiat Clin, I-40123 Bologna, Italy;(3)Univ Florence, Dept Pharmaceut Sci, I-50121 Florence, Italy |
推荐引用方式 GB/T 7714 | Raggi, MA,Mandrioli, R,Casamenti, G,et al. Determination of reboxetine, a recent antidepressant drug, in human plasma by means of two high-performance liquid chromatography methods[C]:ELSEVIER SCIENCE BV,2002:23-33. |
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