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荒漠和草原生态系统丛枝菌根真菌多样性和群落结构比较研究
其他题名Differences in arbuscular mycorrhizal fungi diversity and community structure between desert and steppe ecosystems.
李雪静
出版年2017
学位类型硕士
导师陈保冬
学位授予单位中国科学院大学
中文摘要丛枝菌根真菌(arbuscular mycorrhizal fungi, AMF)是自然生态系统和农业生态系统中分布最为广泛的土壤真菌,能够与绝大多数陆地植物形成互惠共生体并具有重要的生态功能。以我国内蒙古地带性植被区及甘肃、青海非地带性植被区荒漠和草原生态系统为研究对象,基于Illumina Miseq高通量测序平台比较荒漠和草原土壤AMF多样性和群落结构的差异,结合环境因子探讨其主要影响因素,以期为AMF在草原荒漠化生态恢复中的应用提供理论基础。基于高通量数据分析共得到159个AMF-OTUs,分属于1门1纲3目5科8属,其中Glomus属在荒漠和草原土壤中均为优势属。地带性植被区和非地带性植被区的草原土壤AMF丰富度、香农多样性、系统发育α多样性都显著高于荒漠。地带性植被区的荒漠和草原土壤AMF群落结构有显著差异,而非地带性植被区荒漠和草原土壤AMF群落结构差异不显著。相关分析揭示环境因子与土壤AMF群落结构和多样性的相关关系,变差分解的结果进一步表明土壤理化因子和气候因子对AMF群落结构的解释率比宿主植物和地理距离要高,荒漠和草原土壤AMF群落结构主要受土壤理化因子和气候因子形成的环境过滤的影响。
英文摘要Arbuscular mycorrhizal fungi (AMF) is ubiquitous soil fungi in natural and agricultural ecosystems, which can form symbiotic associations with the majority of terrestrial plants and have important ecological functions. To compare the AMF communities between desert and steppe ecosystems, and to reveal the key influencing factors for AMF diversity, we collected rhizosphere soil samples from both zonal and non-zonal desert and steppe in Inner Mongolia, Gansu and Qinghai provinces, China. The community structure of the soil AMF was investigated using Illumina Miseq high-throughput sequencing platform. In total, 159 AMF operational taxonomic units (OTUs) were detected, belonging to 1 class, 3 orders, 5 families and 8 genus, among which, Glomus was the dominant genera in both desert and steppe ecosystems. The AMF richness, Shannon diversity, phylogenetic diversity in steppe was higher than those in desert (both zonal and non-zonal vegetation regions). A remarkable difference in AMF community structure between desert and steppe ecosystems was noted in zonal vegetation region. However, there was no significant difference in AMF community structure between non-zonal desert and steppe. Correlation analysis clearly reflected the relationship between environmental factors and AMF community structure and diversity, and variation partitioning showed that edaphic properties and climate factor were more important than host plants and the geographic distance in determining AMF community structure. In summary, the soil AMF communities of desert and steppe were mainly shaped by environmental filtering including edaphic properties and climate factors. This study provided supports for the potential use of AMF for ecological control of grassland desertification.
中文关键词荒漠 ; 草原 ; AMF ; 环境因子 ; 群落结构
英文关键词desert steppe arbuscular mycorrhizal fungi (AMF) environmental factors community structure
语种中文
国家中国
来源学科分类生态学
来源机构中国科学院生态环境研究中心
资源类型学位论文
条目标识符http://119.78.100.177/qdio/handle/2XILL650/287911
推荐引用方式
GB/T 7714
李雪静. 荒漠和草原生态系统丛枝菌根真菌多样性和群落结构比较研究[D]. 中国科学院大学,2017.
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