Knowledge Resource Center for Ecological Environment in Arid Area
| 逆境胁迫及离子注入对甘草幼苗生长和保护酶系统的影响 | |
| 其他题名 | Effect of Adversity Stress and Ion Beam Implantation on the Growth and Protective Enzyme System of Liquorice |
| 潘燕 | |
| 出版年 | 2005 |
| 学位类型 | 硕士 |
| 导师 | 吴李君 |
| 学位授予单位 | 中国科学院合肥物质科学研究院 |
| 中文摘要 | 甘草是维护我国西部荒漠半荒漠草原地区生态环境的重要植物，现阶段有关于卞卜草的研究多集中于其人工栽培方面，药效药理方面以及药性物质的提取方面，而研究甘草的抗熟性能，这在国内外尚不多见。本文对甘草抗旱耐盐机理以及低能氮离子束注入对一甘草幼苗抗旱性能的影响进行了初步研究和探讨。本论文中，以卞卜草幼苗为研究材料，分别用聚乙二醇（PEG6000）和NaCI模拟干旱和盐汝对其进行根际胁迫处理，测定了胁迫过程中几种保护酶（SOD、P〕D、CA灼的活性及丙二醛（Mevalondialdehyde,MDA）含量的动态变化，同时用电泳方法测定千早和盐胁迫条件下泪-草幼苗SOD同工酶谱的变化情况。结果表明干旱和盐渍均对植物造成渗透伤害，表现为胁迫条件下甘草幼苗脂质过氧化程度增加。干旱和盐胁迫下甘草幼苗超氧化幼苗超氧化物歧化酶SOD和过氧化物酶POD活性增强，而过氧化氛酶CAT活性下降。同工酶电泳结果显示当幼苗受到2％NaCI胁迫处理时，随胁迫时间的延民一条新的MnSOD条带被诱导出来，由于SOD作用的产物是H_2O_2这可能间接导致了过氧化氢酶活的下降。相关性分析结果显示在干旱和盐胁迫下SOD砰海活的变化趋势有所不同，但POD及CAT酶活的变化趋势十分相似，在干旱胁迫下，随着胁迫时间的延长，采用上述两个剂量的离子束注入后萌发的幼苗MDA含量明显低于对照。酶活结果显示，在干旱胁迫后期，剂量4．6只1016N丫cm，处理后的甘草幼苗超氧化物歧化酶（Stlpoloxide di－smLltse，SOD）和过氧化氢酶（Catalase,CAT）活性都高于对照，说明该注入剂量处理-甘草种有助于提高甘草幼苗的抗旱性能，SOD同工酶结果显示注入组发生酶带丢失现象，也证明该剂量的离子注入对干早胁迫下同工酶谱带有所影响。 |
| 英文摘要 | Liquorice is one of the important stress-resistant plants which maintain the ecological environment of desert or semi-desert in northwest China. These plants are largely studied at their artificial culturing, pharmacodynamics and extraction of drug properties, but are overlooked and poorly studied at the biological and biochemical levels. The effects of salinity and drought on the antioxidative system (SOD, POD, CAT) were studied in liquorice sted\\ix\\gs(Glycyrrhiza uralensis Fisch). The results showed that salt and drought stresses both could induce oxidative stresses, as indicated by the increase level of lipid peroxidation. The activities of SOD and POD were up-regulated by salt and drought stress, while CAT activity decreased. An additional MnSOD isoenzyme was also detected in liquorice subjected to 2%NaCl stress, which may be response for the drastic decline of CAT activity when seedlings are exposed to 2%NaCl stress. The data also showed that although the activity of SOD was differentially influenced by drought and salinity, the changes of antioxidant enzyme activities subjected to drought stress follow a pattern similar to that subjected to salt stress, indicating that similar defensive systems might involve in oxidative stress injury in liquorice. The effect of N+ ion implantation on the antioxidative system (SOD> CAT)and MDA content of liquorice seedlings under PEG-induced drought stress was also investigated in this study. The results showed survival ratio of liquorice seeds exhibited saddle-shaped curve. When the dose was 4.1xlO16 N+/cm2, the survival ratio was the lowest, while the survival ratio was higher than that of control when the dose increased to 4.6x1016 N+/cm2. MDA measurement pointed out that the contents were significantly lower in seedlings irradiated by N+ ion beams of 4.6xlO16 N+/crn2 dose when compared to control. The data also showed that SOD and CAT activity of irradiated seedlings by dose of 4.6><1016 N+/cm2 ion beam were higher than control, indicating that this implantation was useful in the enhancement of drought resistance of liquorice seedlings. Result of SOD isoform pattern also demonstrates the biological effects of N+ ion implantation. |
| 中文关键词 | 甘草幼苗 ; 保护酶 ; 干旱胁迫 ; 盐胁迫 |
| 英文关键词 | liquorice seedlings protective enzymes drought stress salt stress |
| 语种 | 中文 |
| 国家 | 中国 |
| 来源机构 | 中国科学院合肥物质科学研究院 |
| 资源类型 | 学位论文 |
| 条目标识符 | http://119.78.100.177/qdio/handle/2XILL650/286428 |
| 推荐引用方式 GB/T 7714 | 潘燕. 逆境胁迫及离子注入对甘草幼苗生长和保护酶系统的影响[D]. 中国科学院合肥物质科学研究院,2005. |
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