干旱区生态环境知识资源中心(Arid): Reconstruction of a Bacterial Genome from DNA Cassettes

Arid

DOI	10.2172/1041049
报告编号	DOE/ER63453-1
来源ID	OSTI_ID: 1041049
	Reconstruction of a Bacterial Genome from DNA Cassettes
	Christopher Dupont; John Glass; Laura Sheahan; Shibu Yooseph; Lisa Zeigler Allen; Mathangi Thiagarajan; Andrew Allen; Robert Friedman; J. Craig Venter
英文摘要	This basic research program comprised two major areas: (1) acquisition and analysis of marine microbial metagenomic data and development of genomic analysis tools for broad, external community use; (2) development of a minimal bacterial genome. Our Marine Metagenomic Diversity effort generated and analyzed shotgun sequencing data from microbial communities sampled from over 250 sites around the world. About 40% of the 26 Gbp of sequence data has been made publicly available to date with a complete release anticipated in six months. Our results and those mining the deposited data have revealed a vast diversity of genes coding for critical metabolic processes whose phylogenetic and geographic distributions will enable a deeper understanding of carbon and nutrient cycling, microbial ecology, and rapid rate evolutionary processes such as horizontal gene transfer by viruses and plasmids. A global assembly of the generated dataset resulted in a massive set (5Gbp) of genome fragments that provide context to the majority of the generated data that originated from uncultivated organisms. Our Synthetic Biology team has made significant progress towards the goal of synthesizing a minimal mycoplasma genome that will have all of the machinery for independent life. This project, once completed, will provide fundamentally new knowledge about requirements for microbial life and help to lay a basic research foundation for developing microbiological approaches to bioenergy.
出版年	2011
报告类型	Technical Report
语种	英语
国家	美国
来源学科分类	59 BASIC BIOLOGICAL SCIENCES ; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE ; BIOLOGY ; CARBON ; COMMUNITIES ; DNA ; ECOLOGY ; GENES ; MACHINERY ; MINING ; MYCOPLASMA ; NUTRIENTS ; PLASMIDS ; RESEARCH PROGRAMS ; VIRUSES
URL	http://www.osti.gov/scitech/servlets/purl/1041049
资源类型	科技报告
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/270389
推荐引用方式 GB/T 7714	Christopher Dupont,John Glass,Laura Sheahan,et al. Reconstruction of a Bacterial Genome from DNA Cassettes,2011.