干旱区生态环境知识资源中心(Arid): Field calibration of soil-core microcosms for evaluating fate and effects of genetically engineered microorganisms in terrestrial ecosystems

Arid

DOI	10.2172/5708022
报告编号	PNL-7667
来源ID	OSTI_ID: 5708022
	Field calibration of soil-core microcosms for evaluating fate and effects of genetically engineered microorganisms in terrestrial ecosystems
	Bolton, H Jr; Fredrickson, J K; Bentjen, S A; Workman, D J; Li, S W; Thomas, J M
英文摘要	Pacific Northwest Laboratory compared intact soil-core microcosms and the field for ecosystem structural and functional properties after the introduction of a model genetically engineered microorganism (GEM). This project used two distinct microbial types as model GEMs, Gram-negative Pseudomonas sp. RC1, which was an aggressive root colonizer, and Gram-positive Streptomyces lividans TK24. The model GEMs were added to surface soil in separate studies, with RC1 studied throughout the growth of winter wheat (Triticum aestivum), while TK24 was studied throughout a ten month period. Also, RC1 was used in studies conducted during two consecutive field seasons (1988 to 1990) to determine how year-to-year field variability influenced the calibration of microcosms with the field. The main conclusions of this research were that intact soil-core microcosms can be useful to simulate the field for studies of microbial fate and effects on ecosystem structural and functional properties. In general, microcosms in the growth chamber, which simulated average field variations, were similar to the field for most parameters or differences could be attributed to the great extremes in temperature that occurred in the field compared to the microcosms. Better controls of environmental variables including temperature and moisture will be necessary to more closely simulate the field for future use of microcosms for risk assessment. 126 refs., 13 figs., 12 tabs.
出版年	1991
报告类型	Technical Report
语种	英语
国家	美国
来源学科分类	59 BASIC BIOLOGICAL SCIENCES ; 54 ENVIRONMENTAL SCIENCES ; MICROCOSMS ; CALIBRATION ; PSEUDOMONAS ; GENETIC ENGINEERING ; STREPTOMYCES ; WHEAT ; PLANT GROWTH ; ANNUAL VARIATIONS ; BATTELLE PACIFIC NORTHWEST LABORATORIES ; EXPERIMENTAL DATA ; SIMULATION ; SOILS ; TERRESTRIAL ECOSYSTEMS ; BACTERIA ; CEREALS ; DATA ; ECOSYSTEMS ; GRASS ; GROWTH ; INFORMATION ; LILIOPSIDA ; MAGNOLIOPHYTA ; MICROORGANISMS ; NATIONAL ORGANIZATIONS ; NUMERICAL DATA ; PLANTS ; US DOE ; US ERDA ; US ORGANIZATIONS ; VARIATIONS 550200* -- Biochemistry ; 540210 -- Environment, Terrestrial-- Basic Studies-- (1990-)
URL	http://www.osti.gov/scitech/servlets/purl/5708022
资源类型	科技报告
条目标识符	http://119.78.100.177/qdio/handle/2XILL650/264431
推荐引用方式 GB/T 7714	Bolton, H Jr,Fredrickson, J K,Bentjen, S A,et al. Field calibration of soil-core microcosms for evaluating fate and effects of genetically engineered microorganisms in terrestrial ecosystems,1991.